Expression of organic anion transporters 1 and 3 in the ovine fetal brain during the latter half of gestation.
Expression of organic anion transporters 1 and 3 in the ovine fetal brain during the latter half of gestation.
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DOI：
10.1016/j.neulet.2010.08.008
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发表时间：
2010-10-22
影响因子：
2.5
通讯作者：
Wood CE
中科院分区：
文献类型：
--
作者：
Cousins R;Wood CE
胎儿大脑的发育和成熟对于子宫内的稳态、对胎儿应激的反应以及反刍动物对出生时间的控制至关重要。绵羊和人类一样，胎盘分泌雌激素和其他信号分子进入胎儿和母体的血液，这些分子穿过血脑屏障的进出很可能是由转运蛋白促进的。本研究的目的是测试的假设，羊胎脑表达有机阴离子转运蛋白，这些转运蛋白的表达作为脑区和胎龄的函数而变化。在妊娠80、100、120、130、145天处死胎羊和新生羊时以及出生后第1天（羊在妊娠约147天分娩），采集脑和垂体。处理下丘脑、延髓脑干、小脑和垂体用于mRNA提取和cDNA合成（4 - 5只/组）。OAT1和OAT3表达的实时PCR分析显示，这两种基因在所有测试的组织中均有显著表达。在下丘脑和小脑，有一个或两个基因的表达在统计学上显着增加对妊娠结束。在延髓脑干和垂体中，表达水平相对不变，因为随着发育年龄的变化没有统计学意义。我们的结论是，羊胎脑表达OAT 1和OAT 3，表达模式表明这些转运蛋白在胎儿大脑发育的生理作用越来越大，胎儿接近自然分娩的时间。
Development and maturation of the fetal brain is critical for homeostasis in utero, responsiveness to fetal stress and, in ruminants, control of the timing of birth. In the sheep, as in the human, the placenta secretes estrogen and other signaling molecules into both the fetal and maternal blood, molecules whose entry or exit across the blood-brain barrier is likely to be facilitated by transporters. The purpose of this study was to test the hypothesis that the ovine fetal brain expresses organic anion transporters, and that the expression of these transporters varies as a function of brain region and fetal gestational age. Brains and pituitaries were collected at the time of sacrifice from fetal and newborn sheep at 80, 100, 120, 130, 145 days gestation and on the first day of postnatal life (parturition in sheep is at approximately 147 days gestation). Hypothalamus, medullary brainstem, cerebellum, and pituitary were processed for mRNA extraction and synthesis of cDNA (4–5/group). Real-time PCR analysis of OAT1 and OAT3 expression revealed significant expression of both genes in all of the tissues tested. In hypothalamus and cerebellum, there were statistically significant increases in the expression of one or both genes towards the end of gestation. In medullary brainstem and pituitary, the levels of expression were relatively unchanged as there were no statistically significant changes with developmental age. We conclude that the ovine fetal brain expresses both OAT1 and OAT3, that the pattern of expression suggests an increasing role for these transporters in the physiology of the developing fetal brain as the fetus nears the time of spontaneous parturition.
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DOI：
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发表时间：
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影响因子：
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作者：
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发表时间：
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影响因子：
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