Rhesus monkey rhadinovirus: a model for the study of KSHV.
Rhesus monkey rhadinovirus: a model for the study of KSHV.
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恒河猴鼻病毒：KSHV 研究模型。
DOI：
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发表时间：
2007
影响因子：
--
通讯作者：
D. Kedes
中科院分区：
文献类型：
--
作者：
Christine M. O'Connor;D. Kedes
恒河猴狂犬病病毒(RRV)是人类病原体卡波西肉瘤相关疱疹病毒(KSHV)的亲缘关系最近的病毒之一，KSHV是一种伽马-2疱疹病毒，是与免疫抑制相关的三种恶性肿瘤的病原体。与KSHV不同，RRV在培养中表现出强劲的裂解期生长，复制到高滴度，因此有望成为研究灵长类伽马疱疹病毒裂解基因转录以及病毒结构、组装和蛋白质组学的有效模型。最近，研究人员设计了RRV感染的互补潜伏系统，从而也能够表征更受限的潜伏转录程序。将RRV作为灵长类伽马疱疹病毒模型的另一个好处是，它高效的裂解生长使基因操作比人类同行更容易。利用这一特性，实验室已经开始产生突变的RRV，为未来研究单个病毒基因的功能奠定了基础。最后，恒河猴支持RRV的实验性感染，提供了一个自然的体内感染模型，而类似的非人类系统仍然对KSHV的长期感染具有抵抗力。最近，RRV和一种猴免疫缺陷病毒(SIV)的双重感染导致了一种淋巴增殖性疾病(LPD)，使人想起多中心Castleman病(MCD)--免疫抑制患者亚群中KSHV感染的临床表现。简而言之，RRV与KSHV具有高度的同源性，但更容易在体外和体内进行实验操作。综上所述，这些特性确保了它目前作为KSHV生物学和感染最相关的病毒模型之一的地位。
Rhesus monkey rhadinovirus (RRV) is one of the closest phylogenetic relatives to the human pathogen Kaposi sarcoma-associated herpesvirus (KSHV)-a gamma-2 herpesvirus and the etiologic agent of three malignancies associated with immunosuppression. In contrast to KSHV, RRV displays robust lytic-phase growth in culture, replicating to high titer, and therefore holds promise as an effective model for studying primate gammaherpesvirus lytic gene transcription as well as virion structure, assembly, and proteomics. More recently, investigators have devised complementary latent systems of RRV infection, thereby also enabling the characterization of the more restricted latent transcriptional program. Another benefit of working with RRV as a primate gammaherpesvirus model is that its efficient lytic growth makes genetic manipulation easier than that in its human counterpart. Exploiting this quality, laboratories have already begun to generate mutant RRV, setting the stage for future work investigating the function of individual viral genes. Finally, rhesus macaques support experimental infection with RRV, providing a natural in vivo model of infection, while similar nonhuman systems have remained resistant to prolonged KSHV infection. Recently, dual infection with RRV and a strain of simian immunodeficiency virus (SIV) has led to a lymphoproliferative disorder (LPD) reminiscent of multicentric Castleman disease (MCD)--a clinical manifestation of KSHV infection in a subset of immunosuppressed patients. RRV, in short, shows a high degree of homology with KSHV yet is more amenable to experimental manipulation both in vitro and in vivo. Taken together, these qualities ensure its current position as one of the most relevant viral models of KSHV biology and infection.
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