Mice lacking all of the Skint family genes
Mice lacking all of the Skint family genes
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DOI：
10.1093/intimm/dxy030
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发表时间：
2018-06
影响因子：
4.4
通讯作者：
Tomoya Narita;T. Nitta;Sachiko Nitta;T. Okamura;H. Takayanagi
中科院分区：
文献类型：
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作者：
Tomoya Narita;T. Nitta;Sachiko Nitta;T. Okamura;H. Takayanagi
γδT细胞在胸腺中发育，并在保护免受感染和肿瘤发展方面发挥重要作用，但胸腺微环境支持γδT细胞分化的机制仍不清楚。Skint 1是一种在胸腺上皮细胞中表达的B7相关蛋白，对小鼠Vγ5Vδ1 γδT细胞的发育至关重要。小鼠Skint家族由11个成员组成，Skint 1 -11。在这里，我们产生了突变小鼠，缺乏包含所有Skint基因的整个基因组区域。这些小鼠在胸腺和皮肤中表现出Vγ 5 V δ1 γδT细胞的显著减少，但令人惊讶的是，其他γδT细胞亚群和白细胞包括αβT、B和骨髓细胞的发育正常。这种表型与Skint 1缺陷小鼠的表型基本相同。这些结果表明，Skint家族在调节Vγ 5 V δ1 γδT细胞的发育中发挥独特的功能，并与其他白细胞的发育有关。
γδT cells develop in the thymus and play important roles in protection against infection and tumor development, but the mechanisms by which the thymic microenvironment supports γδT cell differentiation remain largely unclear. Skint1, a B7-related protein expressed in thymic epithelial cells, was shown to be essential for the development of mouse Vγ5Vδ1 γδT cells. The Skint family in mouse consists of 11 members, Skint1-11. Here we generated mutant mice lacking the entire genomic region that contains all of the Skint genes. These mice exhibited a marked reduction of Vγ5Vδ1 γδT cells in the thymus and skin, but surprisingly, had normal development of other γδT cell subsets and leukocytes including αβT, B and myeloid cells. This phenotype is essentially identical to that of Skint1-deficient mice. These results indicate that the Skint family exerts an exclusive function in regulating the development of Vγ5Vδ1 γδT cells and is dispensable for development of other leukocytes.