Facilitated Neurogenesis in the Developing Hippocampus After Intake of Theanine, an Amino Acid in Tea Leaves, and Object Recognition Memory
Facilitated Neurogenesis in the Developing Hippocampus After Intake of Theanine, an Amino Acid in Tea Leaves, and Object Recognition Memory
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DOI：
10.1007/s10571-011-9707-0
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发表时间：
2011-10-01
影响因子：
4
通讯作者：
Yokogoshi, Hidehiko
中科院分区：
文献类型：
--
作者：
Takeda, Atsushi;Sakamoto, Kazuhiro;Yokogoshi, Hidehiko
茶氨酸是绿色茶中的主要氨基酸成分之一。在这项研究中，认知功能和相关的机制进行了检查，在茶氨酸管理的年轻大鼠。新生大鼠通过母鼠喂食茶氨酸，母鼠喂食含0.3%茶氨酸的水，断奶后再喂食含0.3%茶氨酸的水。在开始给予茶氨酸后6周，脑中的茶氨酸水平低于可检测限。茶氨酸管理没有影响运动活动在开放领域的测试。然而，饲养行为显着增加，在茶氨酸管理的大鼠，这表明，探索活动增加茶氨酸摄入量。此外，对象识别记忆增强茶氨酸管理的大鼠。在旷场测试中探索活动的增加似乎与茶氨酸给药后物体识别记忆的增强有关。另一方面，长时程增强（LTP）诱导在穿通通路颗粒细胞突触没有改变茶氨酸管理。为了检查海马神经发生，在开始给予茶氨酸3周后向大鼠注射BrdU，此时脑源性神经营养因子（BDNF）水平显著升高。茶氨酸摄入量显着增加的BrdU-，Ki 67-，和DCX-标记的细胞在颗粒细胞层的数量6周后，开始茶氨酸管理。这项研究表明，0.3%的茶氨酸管理促进神经发生在发育中的海马，其次是增强识别记忆。茶氨酸的摄入可能有利于海马功能的生后发育。
Theanine, gamma-glutamylethylamide, is one of the major amino acid components in green tea. In this study, cognitive function and the related mechanism were examined in theanine-administered young rats. Newborn rats were fed theanine through dams, which were fed water containing 0.3% theanine, and then fed water containing 0.3% theanine after weaning. Theanine level in the brain was under the detectable limit 6 weeks after the start of theanine administration. Theanine administration did not influence locomotor activity in the open-field test. However, rearing behavior was significantly increased in theanine-administered rats, suggesting that exploratory activity is increased by theanine intake. Furthermore, object recognition memory was enhanced in theanine-administered rats. The increase in exploratory activity in the open-field test seems to be associated with the enhanced object recognition memory after theanine administration. On the other hand, long-term potentiation (LTP) induction at the perforant path-granule cell synapse was not changed by theanine administration. To check hippocampal neurogenesis, BrdU was injected into rats 3 weeks after the start of theanine administration, and brain-derived neurotropic factor (BDNF) level was significantly increased at this time. Theanine intake significantly increased the number of BrdU-, Ki67-, and DCX-labeled cells in the granule cell layer 6 weeks after the start of theanine administration. This study indicates that 0.3% theanine administration facilitates neurogenesis in the developing hippocampus followed by enhanced recognition memory. Theanine intake may be of benefit to the postnatal development of hippocampal function.