BRAIN PROTEINS IN PLANTS - AN ARABIDOPSIS HOMOLOG TO NEUROTRANSMITTER PATHWAY ACTIVATORS IS PART OF A DNA-BINDING COMPLEX
BRAIN PROTEINS IN PLANTS - AN ARABIDOPSIS HOMOLOG TO NEUROTRANSMITTER PATHWAY ACTIVATORS IS PART OF A DNA-BINDING COMPLEX
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DOI：
10.1073/pnas.89.23.11490
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发表时间：
1992-12-01
影响因子：
11.1
通讯作者：
FERL, RJ
中科院分区：
文献类型：
--
作者：
LU, GH;DELISLE, AJ;FERL, RJ
G盒是一个具有顺式作用的DNA调控元件，存在于几个看似无关的植物基因的启动子中，包括拟南芥的乙醇脱氢酶（Adh）基因。使用单克隆抗体的筛选方法加上电泳迁移率变动分析，我们已经分离出一个cDNA克隆编码的蛋白质，是在体外蛋白质/G盒复合物的一部分。衍生的氨基酸序列与哺乳动物脑中的一类蛋白质同源，这些蛋白质被描述为蛋白激酶C抑制剂和酪氨酸和色氨酸羟化酶的激活剂，酪氨酸和色氨酸羟化酶是导致儿茶酚胺和5-羟色胺的途径中的限速酶。该调节蛋白家族的同源成员在植物中被发现并且与转录调节元件结合的事实表明这些蛋白具有更广泛的作用。
The G box is a well-characterized cis-acting DNA regulatory element found in the promoters of several seemingly unrelated plant genes, including the alcohol dehydrogenase (Adh) gene of Arabidopsis thaliana. Using a monoclonal antibody screening approach coupled with electrophoretic mobility shift assays; we have isolated a cDNA clone encoding a protein that is part of the in vitro protein/G box complex. The derived amino acid sequence is homologous to a class of proteins in mammalian brains described as protein kinase C inhibitors and as activators of tyrosine and tryptophan hydroxylases, the rate-limiting enzymes in the pathways leading to the catecholamines and serotonin. The fact that a homologous member of this regulatory protein family is found in plants and is associated with binding to transcriptional regulatory elements suggests a much wider role for these proteins.