Boundary mode frictional properties of engineered cartilaginous tissues
Boundary mode frictional properties of engineered cartilaginous tissues
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DOI：
10.22203/ecm.v014a02
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发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
Bonassar, L. J.
中科院分区：
文献类型：
--
作者：
Gleghorn, J. P.;Jones, A. R. C.;Bonassar, L. J.
尽管润滑是关节软骨的主要功能，但关于软骨工程组织的摩擦特性的信息很少。在边界润滑中，软骨摩擦特性的一种生物化学介质，润滑素，已被证明是由软骨细胞-水凝胶结构分泌的。在目前的研究中，我们利用藻酸盐培养中的关节软骨细胞(CON)、半月板纤维软骨细胞(MEN)和间充质干细胞(MSC)来确定润滑油的定位和固有的边界润滑摩擦系数。此外，我们还研究了这些组织被滑液润滑的能力以及这种润滑的可逆性。细胞-藻酸盐构建物培养6周以上，用ELISA法检测培养液中润滑素的释放，用免疫组织化学(IHC)方法分析构建体中润滑素的定位。在定制的摩擦仪中测试工程组织在边界润滑模式下的平衡摩擦系数(Mu(Eq))，然后与马滑液(SF)孵育，然后在1.5M的氯化钠中提取。MSCs释放的润滑剂是CON或MAN培养的10倍。IHC分析显示，在培养6周时，没有将润滑素定位于藻酸盐，工程化构建物在培养6周时最小的焦点染色，以及当外源性SF处理时，所有工程化组织都有定位润滑素的能力。摩擦特性表明，所有工程组织在培养过程中的Mu(Eq)没有差异，而在SF中孵育的所有组织的Mu(Eq)随培养时间的延长而降低，而提取润滑剂会导致所有工程组织失去润滑性。
Despite the fact that lubrication is a primary function of articular cartilage, there is little information on the frictional properties of cartilaginous engineered tissues. A biochemical mediator of cartilage frictional properties in boundary lubrication, lubricin, has been shown to be secreted from chondrocyte-hydrogel constructs. In the current studies we utilized articular chondrocytes (CON), meniscal fibrochondrocytes (MEN), and mesenchymal stem cells (MSC) in alginate cultures to determine lubricin localization and the inherent boundary lubrication friction coefficient. Additionally, we investigated the ability of these tissues to be lubricated by synovial fluid and the reversibility of this lubrication. Cell-alginate constructs were cultured over six weeks, culture medium assayed for lubricin release by ELISA and constructs analyzed with immunohistochemical (IHC) methods to investigate the localization of lubricin. Engineered tissues were tested in a custom friction instrument to determine the equilibrium friction coefficient (mu(eq)) in boundary lubrication mode, following incubation with equine synovial fluid (SF), and subsequent extraction in 1.5M NaCl. MSCs released 10 fold more lubricin than CON or MEN cultures. IHC analysis showed no localization of lubricin to alginate, minimal focal staining of engineered constructs at six weeks in culture, and the ability of all engineered tissues to localize lubricin when exogenously treated with SF. Frictional characterization showed no difference in mu(eq) over culture for all engineered tissues, while incubation in SF decreased mu(eq) for all tissues over culture duration, and extraction of lubricin resulted in a loss of lubrication of all engineered tissues.