Transcriptional responses in mouse lungs induced by vaccination with Mycobacterium bovis BCG and infection with Mycobacterium tuberculosis
Transcriptional responses in mouse lungs induced by vaccination with Mycobacterium bovis BCG and infection with Mycobacterium tuberculosis
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DOI：
10.1016/j.micinf.2005.06.015
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发表时间：
2006-01-01
影响因子：
5.8
通讯作者：
Kaufmann, SHE
中科院分区：
文献类型：
--
作者：
Mollenkopf, HJ;Hahnke, K;Kaufmann, SHE
转录组分析能够评估整个组织和器官在病理过程中的特征变化。我们分析了感染结核分枝杆菌或接种牛分枝杆菌卡介苗（BCG）的小鼠肺部的基因表达谱。我们比较了高剂量全身和低剂量气溶胶结核分枝杆菌感染以及全身卡介苗接种。使用定制的“原位”合成 60 聚体寡核苷酸微阵列在感染/疫苗接种后第 1 天和第 30 天分析肺部表达谱，重点关注免疫相关基因。在第 1 天，少数基因受到差异调节，而在第 30 天，在肺中发现了离散的表达模式。结核分枝杆菌感染和卡介苗接种之间的差异基因表达谱表明自然和疫苗诱导的肺部免疫反应存在差异。全身性和气溶胶结核分枝杆菌感染的共同特征揭示了与干扰素γ（IFNγ）相关或受其控制的基因的优势。我们假设结核分枝杆菌感染后的差异基因表达谱受到肺细胞组成差异的强烈影响，这是由于免疫细胞（主要是中性粒细胞、嗜碱性粒细胞、嗜酸性粒细胞和单核细胞）迁移到感染部位所致。 (c) 2005 年爱思唯尔 SAS。版权所有。
Transcriptome analyses enable the assessment of signature alterations in whole tissues and organs undergoing pathological processes. We analyzed gene expression profiles of lungs from mice infected with Mycobacterium tuberculosis or vaccinated with Mycobacterium bovis bacille Calmette-Guerin (BCG). We compared high-dose systemic and low-dose aerosol M. tuberculosis infections as well as systemic BCG vaccination. Expression profiles in lungs were analyzed at day (d) 1 and d 30 post infection/vaccination using a custom tailored 'in situ' synthesized 60-mer oligonucleotide microarray with focus on immunologically relevant genes. At d 1, a small number of genes were differentially regulated, whereas at d 30, a discrete expression pattern was identified in the lung. Differential gene expression profiles between M. tuberculosis infection and BCG vaccination indicate differences in naturally and vaccine induced pulmonary immune responses. The shared signature of systemic and aerosol M. tuberculosis infection revealed dominance of genes related to or controlled by interferon gamma (IFN gamma). We assume that differential gene expression profiles after M. tuberculosis infection are strongly influenced by differences in cellular composition of the lung due to migration of immune cells, primarily neutrophils, basophils, eosinophils and monocytes to the site of infection. (c) 2005 Elsevier SAS. All rights reserved.