APOPTOSIS IN DOG THYROID-CELLS
APOPTOSIS IN DOG THYROID-CELLS
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DOI：
10.1006/bbrc.1994.1412
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发表时间：
1994-04-15
影响因子：
3.1
通讯作者：
ROBAYE, B
中科院分区：
文献类型：
--
作者：
DREMIER, S;GOLSTEIN, J;ROBAYE, B
细胞凋亡是一种生理性的细胞死亡，已被证明参与了组织的动态平衡以及由于激素剥夺而导致的组织退化。甲状腺细胞群缓慢翻转，细胞丢失补偿有丝分裂能力。在没有促甲状腺激素的情况下，细胞数量会减少。已经研究了细胞凋亡在这种缺失中的可能参与。在本报告中，我们发现，剥夺血清、表皮生长因子和促甲状腺激素会在原代培养的狗甲状腺细胞中触发核小体间DNA片段化和凋亡细胞死亡的形态改变；放线菌酮处理也有同样的效果。这表明甲状腺细胞被赋予了结构性的凋亡程序，后者可能参与了体内观察到的甲状腺退化，而腺体没有充分的生长刺激。(C)1994年学术出版社。
Apoptosis, a physiological cell death, has been shown to be involved in tissue homeostasis as well as in tissue regression due to hormone deprivation. The thyroid cell population slowly turns over, with cell loss compensating mitogenicity. In the absence of thyrotropin, the cell population decreases. The possible involvement of apoptosis in this loss has been studied. We show in this report that deprivation of serum, epidermal growth factor and thyrotropin triggers internucleosomal DNA fragmentation and morphological modifications characteristic of apoptotic cell death in dog thyroid cells in primary culture; cycloheximide treatment has the same effect. This indicates that thyrocytes are endowed with a constitutive apoptosis ''program'' and that the latter might be involved in the thyroid regression observed in vivo in the absence of full growth stimulation of the gland. (C) 1994 Academic Press, Inc.