Analysis of human tear fluid by Fourier transform infrared spectroscopy
Analysis of human tear fluid by Fourier transform infrared spectroscopy
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DOI：
10.1002/bip.20331
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发表时间：
2005-09
期刊：
影响因子：
2.9
通讯作者：
Yasushi Nagase;S. Yoshida;K. Kamiyama
中科院分区：
文献类型：
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作者：
Yasushi Nagase;S. Yoshida;K. Kamiyama
本研究的目的是寻找人泪液成分中有用的光谱因子，以无创地监测眼部理化状况的昼夜变化。所有泪液样品收集与玻璃微毛细管从两个眼睛的三个捐助者和分析傅立叶变换红外光谱与衰减全反射（FTIR-ATR）。我们测量了2852、1735、1546和1242 cm-1处的峰强度，以及二阶导数光谱中这些峰之间的峰强度比。我们发现从右眼和左眼获得的泪液的峰值强度存在显著的昼夜和个体差异。在这些变化中，我们观察到右眼和左眼之间泪液样本的显著变化。在这种情况下，1242（磷酸酯）和2852 cm-1（脂肪酸亚甲基）的右眼泪液的峰强度比增加在下午（1600至1900小时），而左眼泪液没有显着变化。在1242（磷酸酯）和1546 cm-1（酰胺II）之间的比值中，双眼之间未观察到差异。我们的结论是，右眼和左眼泪液之间的生化成分的昼夜变化的差异，可以通过FTIR技术进行非侵入性和非破坏性的监测，这种方法可能是有用的，在未来的泪液诊断。Biopolymers 79：18-27，2005
The purpose of this research is to find some useful spectroscopic factors in human tear fluid contents to monitor diurnal changes of the physicochemical ocular conditions noninvasively. All tear fluid samples were collected with glass microcapillary tubes from both eyes of three donors and analyzed by Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR–ATR). We measured the peak intensities at 2852, 1735, 1546, and 1242 cm–1, and the peak intensity ratios among those peaks in the second derivative spectra. We found significant diurnal and individual variations in those peak intensities for tear fluid obtained from right and left eyes. Among these variations, we observed significant changes in tear samples between right and left eyes. In this case the peak intensity ratio between 1242 (phosphate ester) and 2852 cm–1 (fatty acid methylene) of right eye tear fluid was increased in the afternoon (1600 to 1900 h), while that of left eye tear fluid did not change significantly. In the ratio between 1242 (phosphate ester) and 1546 cm–1 (amide II), the difference was not observed between both eyes. We conclude that the difference in diurnal variations of biochemical constituents between right and left eye tear fluids could be monitored noninvasively and nondestructively by FTIR technique and this method could be useful in the future for tear diagnoses.© 2005 Wiley Periodicals, Inc. Biopolymers 79: 18–27, 2005