Diversity and activity of phosphate bioleaching bacteria from a high-phosphorus iron ore
Diversity and activity of phosphate bioleaching bacteria from a high-phosphorus iron ore
复制标题
DOI：
10.1016/j.hydromet.2008.02.007
复制
发表时间：
2008-06-01
期刊：
影响因子：
4.7
通讯作者：
Garcia-Balboa, C.
中科院分区：
文献类型：
--
作者：
Delvasto, P.;Valverde, A.;Garcia-Balboa, C.
目前世界上许多铁矿石资源含有超过0.08w%的P，使得这种材料不符合制造金属铁和钢的标准。一些细菌可以溶解磷酸盐矿物，这反过来又有助于为各种人类活动（如农业和采矿）开发环境友好的解决方案。当在特定环境中人工接种时，土著微生物通常在适应方面竞争更好，并且比外源微生物造成更少的生态扭曲。以这种方式，本研究的目的是分离和表征与位于巴西地区的米纳斯吉拉斯的高磷铁矿石的溶磷细菌，并确定其溶解磷部分的机制。矿石材料的磷含量为0.18%（w：w），因此，它被认为是高磷铁矿石。在与铁矿石相关的可培养细菌中，四种形态上不同的细菌菌株显示出高的解磷活性。它们通过16 S rRNA基因测序被鉴定为四个不同的种：Leifsonia xyli FeGl 02、Burkholderia cenocepacia FeSu 01、Burkholderia caribensis FeGl 03和Burkholderia ferrariae FeGl 01。除了磷酸三钙[Ca-3（PO 4）（2）]的增溶活性外，还在平板培养中测试了后两种菌株对铍石[AlPO 4]和绿松石[CuAl 6（PO 4）（4）（OH）（x）中心点5 H（2）O]的增溶。虽然这两种菌株都能够在铍矿修正培养基中生长，但都没有表现出增溶活性。也不是B。Caribensis FeG1 03 nor B. ferrariae FeG 101在绿松石改良的培养基中生长。在添加羟基磷灰石[Ca 5（PO 4）（3）（OH）]的培养基中生长的摇瓶培养物中测定了P的溶解速率以及葡萄糖酸、2-酮基葡萄糖酸、乙酸和柠檬酸的产生。这两株菌的溶磷率无明显差异。虽然在肉汤培养基中既没有检测到2-酮葡萄糖酸也没有检测到柠檬酸，但两种菌株都产生葡萄糖酸和乙酸。然而，在B之间观察到葡萄糖酸浓度演变的显著差异。caribensis FeG 1 0 3和B. FerrariaFeGl 01. (C)2008 Elsevier B. V.保留所有权利。
Many of the current world iron ore resources contain over 0.08 w% of P, making this material out-of-standard for the manufacture of metallic iron and steel. Solubilization of phosphatic minerals can be performed by some bacteria, which in turn become Useful in developing environmental friendly solutions for various human activities, such as agriculture and mining. When artificially inoculated in a particular environment, indigenous microorganisms, as a general rule, compete better in terms of adaptation and cause fewer ecological distortions than exogenous microorganisms. In this way, the aims of this study were to isolate and characterize phosphate-solubilizing bacteria associated to a high-phosphorous iron ore located in the Brazilian region of Minas Gerais and to determine the mechanisms by which they solubilize phosphorous moieties. Phosphorus content of the ore material is 0.18% (w:w) and, therefore, it is considered as a high-phosphorus iron ore. Among the culturable bacteria associated to the iron ore, four morphologically distinct bacterial strains showed high phosphate-solubilizing activity-They were identified by 16S rRNA gene sequencing as four different species: Leifsonia xyli FeGl 02, Burkholderia cenocepacia FeSu 01, Burkholderia caribensis FeGl 03 and Burkholderia ferrariae FeGl 01. In addition to tricalcium phosphate [Ca-3(PO4)(2)] solubilization activity, the two later strains were also tested for solubilization of berlinite [AlPO4] and turquoise [CuAl6(PO4)(4)(OH)(x)center dot 5H(2)O] in plate culture. Although both strains were able to growth in the berlinite-amended medium, none showed solubilizing activity. Neither B. caribensis FeGl 03 nor B. ferrariae FeGl 01 grew in the turquoise-amended medium. The rates of P solubilization and production of gluconic acid, 2-ketogluconic acid, acetic acid and citric acid were assayed in flask-shake cultures growing in a medium supplemented with hydroxyapatite [Ca5(PO4)(3)(OH)]. There was no appreciable difference in the rate of P solubilization between these two strains. Although neither 2-ketogluconic nor citric acid were detected in the broth media, both strains produced gluconic acid and acetic acid. However, a remarkable difference in the evolution of the gluconic acid concentration was observed between B. caribensis FeG1 03 and B. ferrariae FeGl 01. (C) 2008 Elsevier B.V. All rights reserved.