Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the Andaman Islands: For cultivation and utility in cosmetic and herbal drug applications
Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the Andaman Islands: For cultivation and utility in cosmetic and herbal drug applications
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DOI：
10.1016/j.indcrop.2010.07.003
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发表时间：
2010-11
影响因子：
5.9
通讯作者：
M. Thomas;Rajani Kurup;A. J. Johnson;S. P. Chandrika;P. J. Mathew;M. Dan;Sabulal Baby
中科院分区：
文献类型：
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作者：
M. Thomas;Rajani Kurup;A. J. Johnson;S. P. Chandrika;P. J. Mathew;M. Dan;Sabulal Baby
积雪草是一种多年生草本植物，在传统医药、草药和化妆品中具有很高的用途。在印度，由于国内和出口需求很高，C。野生亚洲虎种群正以不受控制的速度被过度开发。本研究的目的是确定C.亚洲的一个广泛的地理区域，在印度半岛和安达曼群岛，以强调其高产基因型的商业目的的栽培。六十度。从南印度和安达曼群岛的不同地点收集的亚洲品种在相同的环境条件下在田间基因库中生长。本研究从这些C.通过精确的HPTLC-密度测定法同时估计亚洲品种的加入。C.积雪草（全草）在移动的流动相（4：1：5，v/v）丁醇：乙酸乙酯：水中得到了很好分离的羟基积雪草苷和积雪草苷的双斑点，Rf值分别为0.34±0.02和0.47±0.02。在570 nm处用光密度法评价两种三萜糖苷。在60份材料中，羟基积雪草苷和积雪苷含量最高，为5.67±0.08%（干重）。全株的含量）和1.70± 0.02%。三个C亚洲菜品种仅表现出两种三萜糖苷的不可检测的（接近零）水平。本研究发现了羟基积雪草苷/积雪草苷含量高的优良化学型/基因型，建议对其进行保护和栽培，以利于大规模应用。亚洲菜由于这60株C.在相同的环境条件下生长的积雪苷品种，它们之间羟基积雪草苷/积雪苷含量的变化仅仅是由于它们的遗传组成、表达水平和其它生物合成步骤的变化。本研究未发现羟基积雪草苷/积雪草苷含量与其原始采集地的海平面高度相关。
Centella asiatica is a perennial herb with high utility in traditional medicines, herbal drugs and cosmeceutics. In India, owing to its high domestic and export demands, C. asiatica populations in the wild are overexploited at an uncontrolled rate. The aim of this study was to identify potential genotypes/chemotypes of C. asiatica from a wide geographical region in Peninsular India and the Andaman Islands, so as to emphasize cultivation of its high-yielding genotypes for commercial purposes. Sixty C. asiatica accessions collected from various locations in south India and the Andaman Islands were grown in a Field Gene Bank under identical environmental conditions. Potential ursane-type triterpene glycosides, madecassoside and asiaticoside, in the third generation vegetatively propagated progenies of these C. asiatica accessions were estimated simultaneously by precise HPTLC–densitometry. Methanol extracts of C. asiatica (whole plant) gave well resolved twin spots of both madecassoside and asiaticoside in the mobile phase (4:1:5, v/v) butanol:ethyl acetate:water, at Rfvalues 0.34±0.02 and 0.47±0.02, respectively. Both triterpene glycosides were evaluated densitometrically at 570nm. Of the sixty accessions, highest detected madecassoside and asiaticoside contents were 5.67±0.08% (dry wt. of whole plant) and 1.70±0.02%, respectively. Three C. asiatica accessions showed only non-detectable (near zero) levels of both triterpene glycosides. Elite chemotypes/genotypes with high madecassoside/asiaticoside contents were discovered in this study, recommending their conservation and cultivation for large-scale applications of C. asiatica. Since the vegetatively propagated progenies of these sixty C. asiatica accessions were grown under identical environmental conditions, the variations in madecassoside/asiaticoside content(s) between them are only due to variations in their genetic make-up, expression levels and other biosynthetic steps. No correlation with the sea level heights of their original collection locations and madecassoside/asiaticoside content(s) was found in this study.