Requirement of the poly(A) tail in coronavirus genome replication.
Requirement of the poly(A) tail in coronavirus genome replication.
复制标题
冠状病毒基因组复制中对聚腺苷酸尾的要求。
DOI：
10.1007/978-1-4615-1325-4_68
复制
发表时间：
2001
影响因子：
--
通讯作者：
Hogue,BG
中科院分区：
文献类型：
--
作者：
Spagnolo,JF;Hogue,BG
The 3′ poly (A) tail plays an important, but as yet undefined role in Coronavirus genome replication. To further examine the requirement for the Coronavirus poly(A) tail, we created truncated poly(A) mutant defective interfering (DI) RNAs and observed the effects on replication. Bovine Coronavirus (BCV) and mouse hepatitis Coronavirus A59 (MHV-A59) DI RNAs with tails of 5 or 10 A residues were replicated, albeit at delayed kinetics as compared to DI RNAs with wild type tail lengths (>50 A residues). A BCV DI RNA lacking a poly(A) tail was unable to replicate; however, a MHV DI lacking a tail did replicate following multiple virus passages. Poly(A) tail extension/repair was concurrent with robust replication of the tail mutants. Binding of the host factor poly(A)- binding protein (PABP) appeared to correlate with the ability of DI RNAs to be replicated. Poly(A) tail mutants that were compromised for replication, or that were unable to replicate at all exhibited lessin vitroPABP interaction. The data support the importance of the poly(A) tail in Coronavirus replication and further delineate the minimal requirements for viral genome propagation.
翻译而非编码序列对于冠状病毒小鼠肝炎病毒有缺陷的干扰RNA的有效传播至关重要
影响因子：
5.4
作者：
G. Robbert;Van Der;Most;Willem Luytjes;Saskia Rutjes;Willy J. M. Spaan
通讯作者：
Willy J. M. Spaan
影响因子：
5.4
作者：
DEGROOT, RJ;VANDERMOST, RG;SPAAN, WJM
通讯作者：
SPAAN, WJM