Angiogenesis detected after embolic stroke in rat brain using magnetic resonance T2*WI
Angiogenesis detected after embolic stroke in rat brain using magnetic resonance T2*WI
复制标题
DOI：
10.1161/strokeaha.107.502146
复制
发表时间：
2008-05-01
期刊：
影响因子：
8.3
通讯作者：
Chopp, Michael
中科院分区：
文献类型：
--
作者：
Ding, Guangliang;Jiang, Quan;Chopp, Michael
背景和目的-本研究使用T-2* 加权成像（T2*WI）来测量中风发作后6周内在有或没有西地那非治疗的情况下发生栓塞性中风的大鼠中脑血管生成的时间演变。方法-使雄性Wistar大鼠发生栓塞性中风并用盐水（n = 10）或西地那非（n = 11）治疗，在缺血发作后24小时开始治疗，每天持续7天。在栓塞后24小时和6周内每周使用7特斯拉系统进行T2*WI测量。MRI scannes.Results -使用T2*WI，脑血管生成检测开始于4周和2周后，盐水和西地那非治疗的大鼠栓塞性中风，分别在6周后获得的组织学测量。脑栓塞后血管生成的时间和空间特征的显着差异，脑卒中发作后6周，生理盐水和西地那非治疗的大鼠之间，并确定与T2*WI。MRI渗透性参数K-i补充检测栓塞性卒中大鼠缺血后的血管生成。西地那非治疗的中风大鼠显着增强血管生成，证实histologically.Conclusions - T2*WI可以定量测量血管生成的时间演变在大鼠栓塞性中风。与对照组大鼠相比，西地那非治疗显著增加了中风后6周内治疗动物的血管生成。
Background and Purpose - This study uses T-2* weighted imaging (T2*WI) to measure the temporal evolution of cerebral angiogenesis in rats subjected to embolic stroke up to 6 weeks after stroke onset with or without sildenafil treatment.Method - Male Wistar rats were subjected to embolic stroke and treated with saline (n = 10) or with sildenafil ( n = 11), with treatment initiated at 24 hours and continued daily for 7 days after onset of ischemia. T2*WI measurements were performed at 24 hours after embolization and weekly up to 6 weeks using a 7-Tesla system. Histological measurements were obtained at 6 weeks after MRI scans.Results - Using T2*WI, cerebral angiogenesis was detected starting from 4 weeks and from 2 weeks after onset of embolic stroke in saline and sildenafil treated rats, respectively. Significant differences in the temporal and spatial features of angiogenesis after embolic stroke up to 6 weeks after onset of stroke were found between saline and sildenafil treated rats and were identified with T2*WI. MRI permeability parameter, K-i, complementarily detected angiogenesis after ischemia in embolic stroke rats. Sildenafil treatment of stroke rats significantly enhanced the angiogenesis, as confirmed histologically.Conclusions - T2*WI can quantitatively measure the temporal evolution of angiogenesis in rats subjected to embolic stroke. Compared to control rats, sildenafil treatment significantly increased angiogenesis in treated animals up to 6 weeks after stroke.