Respiratory mechanics in mice: strain and sex specific differences.
Respiratory mechanics in mice: strain and sex specific differences.
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小鼠呼吸力学：应变和性别特异性差异。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
R. Balling
中科院分区：
文献类型：
--
作者：
H. Schulz;C. Johner;G. Eder;A. Ziesenis;P. Reitmeier;J. Heyder;R. Balling
为了评估遗传背景对呼吸力学的贡献，我们开发了一种呼吸机单元来测量小鼠的肺功能参数。我们研究了两个常用的近交系小鼠品系，来自小家鼠（C57 BL/6和C3 HeB/FeJ）和第三个品系，来自小家鼠molossinus [日本花式小鼠1（JF 1）]。该呼吸机可精确执行测量吸气和呼气储备容量、准静态和动态顺应性以及气道阻力所需的不同呼吸操作。结合用于监测气体浓度的质谱仪，进行单次呼吸操作，并获得He-呼吸图，由此确定死腔体积和第三阶段的斜率。在通过腹膜内过量甲苯噻嗪和氯胺酮处死后立即研究了每种品系和每种性别的10只2月龄动物。C3 HeB/FeJ和C57 BL/6表现出相当的肺体积。例如，在雄性C3 HeB/FeJ小鼠中，肺活量（VC）为1072 +/- 79 μ L，吸气储备容量为782 +/- 88 μ L，总肺膨胀时的死腔体积为216 +/- 18 μ L。JF 1的肺体积显著更低（例如VC 611 +/-53微升，P < 0.01），即使当标准化为体重时。在所有三种菌株中，女性的比肺容积显著高于男性，这可能是由于妊娠和哺乳期间的需氧量较高，这两种情况都占据了它们的大部分生命时间。C3 HeB/FeJ中的静态顺应性为64.3 +/- 5.4 microL cmH 2 O-1。在C57 BL/6和JF 1小鼠中，即使与肺体积相关，它也较小。表型变异的遗传与非遗传成分的程度分析显示，混合人群中肺容积和静态顺应性的总变异中至少80%可归因于个体之间的遗传差异。这些差异将在进一步的分离和遗传连锁分析中得到验证。
To assess the contribution of genetic background to respiratory mechanics, we developed a ventilator unit to measure lung function parameters in the mouse. We studied two commonly used inbred mice strains originating from Mus musculus domesticus (C57BL/6 and C3HeB/FeJ) and a third strain derived from Mus musculus molossinus [Japanese fancy mouse 1 (JF1)]. The ventilator allows for accurate performance of the different breathing manoeuvres required for measuring in- and expiratory reserve capacity, quasi-static and dynamic compliance, and airway resistance. In combination with a mass spectrometer for monitoring gas concentrations, single-breath manoeuvres were performed and He-expirograms obtained, from which dead space volume and slope of phase III were determined. From each strain and each sex, 10, 2-month old animals were studied immediately after being killed by an intraperitoneal overdose of xylazine and ketamine. C3HeB/FeJ and C57BL/6 exhibited comparable lung volumes. In male C3HeB/FeJ mice, e.g. vital capacity (VC) was 1072 +/- 79 microL, inspiratory reserve capacity 782 +/- 88 microL, and dead space volume at total lung inflation 216 +/- 18 microL. Lung volumes of JF1 were significantly lower (e.g. VC 611 +/- 53 microL, P < 0.01) even when normalized to body weight. In all three strains, specific lung volumes were significantly higher in females than in males, possibly explained by a higher oxygen demand during pregnancy and lactation, both of which fill most of their life times. Static compliance in C3HeB/FeJ was 64.3 +/- 5.4 microL cmH2O-1. It was smaller in C57BL/6 and JF1 mice, even when related to the lung volume. Analysis of the degree of genetic vs. non-genetic components of the phenotypic variation revealed that at least 80% of the total variation of lung volumes and static compliance in the mixed population is attributable to genetic differences between individuals. These differences will be verified in further studies by segregation and genetic linkage analysis.
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DOI：
10.1165/ajrcmb.22.5.3895
发表时间：
2000-05-01
影响因子：
6.4
作者：
Ohtsuka, Y;Brunson, KJ;Kleeberger, SR
通讯作者：
Kleeberger, SR
DOI：
10.1164/ajrccm.160.4.9806034
发表时间：
1999-10-01
影响因子：
24.7
作者：
Brewer, JP;Kisselgof, AB;Martin, TR
通讯作者：
Martin, TR
DOI：
10.1165/ajrcmb.22.5.3912
发表时间：
2000-05-01
影响因子：
6.4
作者：
Kleeberger, SR;Reddy, S;Jedlicka, AE
通讯作者：
Jedlicka, AE
吸气计时差异与小鼠 3 号染色体存在遗传关联。
DOI：
10.1152/jappl.1998.85.1.360
发表时间：
1998
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Tankersley,CG;DiSilvestre,DA;Jedlicka,AE;Wilkins,HM;Zhang,L
通讯作者：
Zhang,L