EXPRESSION OF UNILATERAL INCOMPATIBILITY IN POLLEN OF LYCOPERSICON-PENNELLII IS DETERMINED BY MAJOR LOCI ON CHROMOSOME-1, CHROMOSOME-6 AND CHROMOSOME-10
EXPRESSION OF UNILATERAL INCOMPATIBILITY IN POLLEN OF LYCOPERSICON-PENNELLII IS DETERMINED BY MAJOR LOCI ON CHROMOSOME-1, CHROMOSOME-6 AND CHROMOSOME-10
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DOI：
10.1007/bf00227314
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发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
DEVERNA, JW
中科院分区：
文献类型：
--
作者：
CHETELAT, RT;DEVERNA, JW
我们以前曾描述过野生茄类植物茄类番茄（Solanum lycopersicoides）通过二倍体或倍半二倍体杂交（后者由茄类番茄的两个基因组和茄类番茄的一个基因组组成）向番茄（lycopersicoides esculentum）的基因渗入。两种属间杂交种均表现花粉不育性，但胚珠可育性。单侧不亲和性阻碍了它们与雄蕊草的直接杂交。野生近缘种pennellii自交亲和的花粉与L. esculentum与S. lycopersicoides杂交的雌蕊亲和。通过将该性状回交到羊尾草中，培育出可用于获得属间杂种后代和研究桥接能力遗传的桥接系。在以秋葵桥系授粉的L. esculentum与S. lycopersicoides杂交后代中，观察到1、6和10号染色体上某些同工酶和RFLP标记优先传递秋葵等位基因。歪斜的分离表明与三个主要的花粉表达相容性位点有关。花粉管生长的观察证实了这一点，表明二倍体属间杂种与雌蕊的亲和性只发生在桥系中，至少杂合在1、6和10号染色体上的pennellii标记。与倍半二倍体杂种的亲和只需要1号染色体和6号染色体位点，表明基因剂量对雌蕊不亲和表达有明显影响。在一个F2小茴香与羽绒花的群体中，1号和10号染色体上的相同标记，以及3、11和12号染色体上的其他标记，均发生了羽绒花等位基因的优先传递，但6号染色体上没有。1号染色体花粉相容性位点位于或靠近s位点，这决定了s等位基因的特异性。结果讨论了现有的遗传模型的单侧不相容，包括可能参与的s位点。
We have previously described gene introgression from the wild nightshade Solanum lycopersicoides into tomato (Lycopersicon esculentum) through the use of either diploid or sesquidiploid hybrids (the latter consisting of two genomes of L. esculentum and one genome of S. lycopersicoides). Both types of intergeneric hybrids display pollen sterility, but workable ovule fertility. Unilateral incompatibility prevents their direct hybridization with staminate L. esculentum. Pollen of a self-compatible form of the related wild species L. pennellii is compatible with pistils of L. esculentum x S. lycopersicoides hybrids. This trait was backcrossed from L. pennellii to L. esculentum in order to develop bridging lines that could be used to obtain progeny from the intergeneric hybrids and to study the inheritance of bridging ability. In progeny of L. esculentum x S. lycopersicoides hybrids pollinated with L. pennellii-derived bridging lines, preferential transmission of L. pennellii alleles was observed for certain isozyme and RFLP markers on chromosomes 1, 6 and 10. The skewed segregations suggest linkage to three major pollen-expressed compatibility loci. This was confirmed by observations of pollen tube growth, which indicated that compatibility with pistils of the diploid intergeneric hybrid occurred only in bridging lines at least heterozygous for the L. pennellii markers on chromosomes 1, 6 and 10. Compatibility with the sesquidiploid hybrid required only the chromosome 1 and 6 loci, indicating an apparent effect of gene dosage on expression of incompatibility in the pistil. In an F2 L. esculentum x L. pennellii population, preferential transmission of L. pennellii alleles was observed for the same markers on chromosomes 1 and 10, as well as other markers on chromosomes 3, 11, and 12, but not 6. The chromosome 1 pollen compatibility locus maps to or near the S-locus, which determines S-allele specificity. The results are discussed in relation to existing genetic models for unilateral incompatibility, including the possible involvement of the S-locus.