Efficient de novo assembly and modification of large DNA fragments
Efficient de novo assembly and modification of large DNA fragments
复制标题
大 DNA 片段的高效从头组装和修饰
DOI：
10.1007/s11427-021-2029-0
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发表时间：
2021-12-16
影响因子：
9.1
通讯作者：
Dai, Junbiao
中科院分区：
文献类型：
--
作者：
Jiang, Shuangying;Tang, Yuanwei;Dai, Junbiao
合成基因组学为病毒和微生物基因组的功能研究提供了新的自下而上的平台。构建大型、千兆位（Gb）大小的高等生物基因组将大大加深我们对遗传蓝图的理解。但是，为了合成和组装这种大规模的基因组，需要开发新的或扩展的方法。在这项研究中，我们开发了一种有效的管道，用于构建大小为100个碱基（kb）或以上的大DNA片段，并描述了一种有效的方法，用于“无疤痕”的组装序列的工程。因此，我们的方法应该为生产长DNA分子提供一个标准框架，这是合成基因组学和代谢工程的关键材料。
Synthetic genomics has provided new bottom-up platforms for the functional study of viral and microbial genomes. The construction of the large, gigabase (Gb)-sized genomes of higher organisms will deepen our understanding of genetic blueprints significantly. But for the synthesis and assembly of such large-scale genomes, the development of new or expanded methods is required. In this study, we develop an efficient pipeline for the construction of large DNA fragments sized 100 kilobases (kb) or above from scratches and describe an efficient method for "scar-free" engineering of the assembled sequences. Our method, therefore, should provide a standard framework for producing long DNA molecules, which are critical materials for synthetic genomics and metabolic engineering.