Development of fetal thymocytes in organ cultures. Effect of interleukin 2
Development of fetal thymocytes in organ cultures. Effect of interleukin 2
复制标题
器官培养中胎儿胸腺细胞的发育。
DOI：
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发表时间：
1987
影响因子：
15.3
通讯作者：
J. Watson
中科院分区：
文献类型：
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作者：
M. Skinner;G. Gros;J. Marbrook;J. Watson
胚胎胸腺细胞主要为Thy-1+、L3T4-、Ly-2-，表达白细胞介素2受体(IL-2)。在胎儿胸腺器官培养中，胸腺细胞的发育一直受到关注。14d胎胸腺培养6d后，胸腺细胞数增加20-40倍，95%为Thy-1+、L3T4+、Ly-2+。14天胎胸腺器官培养中加入IL-2可剂量依赖性地抑制胸腺细胞增殖和Thy-1+、L3T4+、Ly-2+胸腺细胞的出现。IL-2的加入还导致了对同基因和同基因胎儿胸腺细胞和同基因肿瘤靶点具有细胞毒性的细胞群体的出现。虽然导致14天胎儿胸腺细胞表达IL-2受体的事件尚不清楚，但胎儿胸腺器官培养中的IL-2抑制了胎儿胸腺细胞的正常成熟，并提出了这样一个问题：出现的细胞毒细胞是否通过另一种发育途径反映了选择。
Most fetal thymocytes from 14-d mouse embryos are Thy-1+, L3T4-, Ly-2-, and express the receptor for interleukin 2 (IL-2). The development of thymocytes has been followed in fetal thymus organ cultures. When fetal thymus from 14-d embryos were cultured for a 6-d period, thymocytes increased in number 20-40-fold, and 95% became Thy-1+, L3T4+, Ly-2+. The addition of IL-2 to organ cultures of 14-d fetal thymus inhibited, in a dose-dependent manner, cell proliferation and the appearance of Thy-1+, L3T4+, Ly-2+ thymocytes. The addition of IL-2 also resulted in the appearance of a population of cells that were cytotoxic for syngeneic and allogeneic fetal thymocytes and syngeneic tumour targets. While the events that lead to the expression of the IL-2 receptor on 14- d fetal thymocytes are unknown, IL-2 in fetal thymus organ cultures inhibits the normal maturation of fetal thymocytes and raises the question of whether the cytotoxic cells that appear reflect selection through an alternative pathway of development.
小鼠胸腺中淋巴细胞归巢受体表达的个体发育。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Reichert,RA;Jerabek,L;Gallatin,WM;Butcher,EC;Weissman,IL
通讯作者：
Weissman,IL