Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy.
Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy.
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高效液相色谱-傅里叶变换红外光谱法分离测定水环境中的雌激素
DOI：
10.1038/srep32264
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发表时间：
2016-08-31
影响因子：
4.6
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Zheng B;Li W;Li H;Liu L;Lei P;Ge X;Yu Z;Zhou Y
研究了连接高效液相色谱（HPLC）和傅里叶变换红外光谱（FTIR）测定水环境中雌激素的组件，包括加热雾化、溶剂去除、样品沉积、驱动控制、光谱收集、芯片交换、清洗和干燥。结果表明，当雾化温度提高到388 K时，雌激素红外测量中流动相组分（甲醇、H2O、乙腈和NaH2PO4）的干扰被完全消除，分离后得到的红外图谱与标准红外图谱的相似度为0.999。在使用不同 HPLC 进样体积的实验中，在 20ul 进样体积、0.01mg/L BPA 下获得了高度相似的红外光谱，而在 80ul 进样体积下获得了 10ng/L BPA 的有用红外光谱。此外，根据中红外区域红外光谱的峰值强度半定量计算天然水样中的雌激素浓度。
The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H2O, acetonitrile, and NaH2PO4) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region.
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影响因子：
56.9
作者：
TOPSOE, NY
通讯作者：
TOPSOE, NY
影响因子：
3.8
作者：
Gosav, S.;Dinica, R.;Praisler, M.
通讯作者：
Praisler, M.
影响因子：
11.4
作者：
Martin, Sebastien;Shchukarev, Andrey;Boily, Jean-Francois
通讯作者：
Boily, Jean-Francois
DOI：
10.7524/j.issn.0254-6108.2015.08.2015011801
发表时间：
2015-08-01
期刊：
Huanjing Huaxue-Environmental Chemistry
影响因子：
--
作者：
Niu Xiameng;Shi Yali;Cai Yaqi
通讯作者：
Cai Yaqi
影响因子：
7.4
作者：
REEDY, GT;ETTINGER, DG;BOURNE, S
通讯作者：
BOURNE, S