Restriction Endonuclease Analyses of Two Porcine Mycoplasma Deoxyribonucleic Acids: Sequence-Specific Methylation in the Mycoplasma hyopneumoniae Genome?
Restriction Endonuclease Analyses of Two Porcine Mycoplasma Deoxyribonucleic Acids: Sequence-Specific Methylation in the Mycoplasma hyopneumoniae Genome?
复制标题
两种猪支原体脱氧核糖核酸的限制性内切酶分析：猪肺炎支原体基因组中的序列特异性甲基化？
DOI：
10.1099/00207713-34-1-16
复制
发表时间：
1984
影响因子：
2.8
通讯作者：
R. Ross
中科院分区：
文献类型：
--
作者：
H. Chan;R. Ross
猪肺炎支原体和簇状支原体的脱氧核糖核酸可以用限制性内切酶很容易地区分开来。识别富含鸟嘌呤和胞嘧啶的序列的酶，如SmaI和BamHI，特别有用，因为它们产生的脱氧核糖核酸片段相对较少，可以通过凝胶电泳法进行分辨。此外，我们还发现猪肺炎分枝杆菌GATC序列中的腺嘌呤核苷酸是甲基化的，而絮状分枝杆菌的腺嘌呤核苷酸不是甲基化的。
The deoxyribonucleic acids of two porcine mycoplasmas, Mycoplasma hyopneumoniae and Mycoplasma flocculare, were distinguished readily with restriction enzymes. Enzymes that recognized guanine-plus-cytosine-rich sequences, such as SmaI and BamHI, were particularly useful since they generated relatively small numbers of deoxyribonucleic acid fragments which could be resolved by gel electrophoresis. In addition, we found that the adenine nucleotide in the GATC sequence of M. hyopneumoniae is methylated, whereas that in M. flocculare is not.