Worm expulsion and mucosal mast cell response induced by repetitive IL-3 administration in Strongyloides ratti-infected nude mice.
Worm expulsion and mucosal mast cell response induced by repetitive IL-3 administration in Strongyloides ratti-infected nude mice.
复制标题
在大鼠圆线虫感染的裸鼠中重复施用 IL-3 诱导蠕虫排出和粘膜肥大细胞反应。
作者：
T. Abe;Yukifumi Nawa
先天性无胸腺nu/nu小鼠初次感染棒状圆线虫后，蠕虫未被排出，肠粘膜肥大细胞（MMC）的数量保持在较低水平。当S.从感染后第5天到第10天，通过重复注射半纯化的IL-3（总共1.4 × 10（5）U）治疗ratti感染的nu/nu小鼠，在第13天观察到粪便中的幼虫排泄（LPG）显著减少。IL-3处理的小鼠小肠中蠕虫的数量显著低于未处理的感染裸鼠。从第4天到第13天的较高剂量IL-3治疗（总计5.8 × 10（5）U）早在第9天就引起了更深刻的LPG减少，尽管直到第13天（该系列实验结束）才观察到LPG完全停止。通过这种较高剂量的IL-3处理，成虫完全从小肠排出，尽管在盲肠中发现少量残留蠕虫，这可以解释从第9天至第13天检测到的持续低水平的LPG。组织学检查显示，IL-3治疗组小鼠小肠MMC的数量，特别是在上皮细胞中，显著高于未治疗组小鼠。
After a primary infection of congenitally athymic nu/nu mice with Strongyloides ratti, worms were not expelled and the number of intestinal mucosal mast cells (MMC) remained at a low level. When S. ratti-infected nu/nu mice were treated by repetitive injections of semi-purified IL-3 from Day 5 to Day 10 post-infection (total 1.4 X 10(5) U), significant reduction of larval excretion in faeces (LPG) was observed on Day 13. The number of adult worms in the small intestine of IL-3-treated mice was significantly lower than that of untreated, infected nude mice. The higher dose of IL-3 treatment from Day 4 to Day 13 (total 5.8 X 10(5) U) caused more profound reduction of LPG as early as Day 9, although complete cessation of LPG was not observed until Day 13, the end of this series of experiments. By this higher dose of IL-3 treatment, adult worms were completely expelled from the small intestine, although a small number of residual worms, which could explain the persistent low level of LPG detected from Day 9 to Day 13, was found in the caecum. Histological examination revealed that the number of MMC, especially in the epithelium, of the small intestine of IL-3-treated mice was significantly higher than that of untreated mice.