GENETIC-ANALYSIS OF CELL MORPHOGENESIS IN FISSION YEAST - A ROLE FOR CASEIN KINASE-II IN THE ESTABLISHMENT OF POLARIZED GROWTH
GENETIC-ANALYSIS OF CELL MORPHOGENESIS IN FISSION YEAST - A ROLE FOR CASEIN KINASE-II IN THE ESTABLISHMENT OF POLARIZED GROWTH
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DOI：
10.1002/j.1460-2075.1994.tb06481.x
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发表时间：
1994-05-01
期刊：
影响因子：
11.4
通讯作者：
NURSE, P
中科院分区：
文献类型：
--
作者：
SNELL, V;NURSE, P
我们发起了一项研究，以确定裂殖酵母裂殖酵母中调节细胞形态发生的基因。已鉴定出五个基因：orb1-orb5，其突变会产生球形细胞，表明无法极化生长。另外两个基因已经被鉴定出来：teal 和 ban1，它们的突变等位基因扰乱了尖端的生长模式，导致细胞呈 T 形和弯曲。在裂殖酵母中，细胞壁沉积位点由肌动蛋白定位确定，肌动蛋白分布和生长模式经历细胞周期阶段特异性重组。对 orb5-19 和细胞分裂前后阻断的各种 cdc 突变体之间构建的双突变体的研究表明，orb5 是胞质分裂后极性生长重建所必需的。这表明突变等位基因 orb5-19 在极化生长的重新启动方面存在缺陷，即使细胞尖端的肌动蛋白重组正常发生。 orb5 编码酪蛋白激酶 II α 的裂殖酵母同源物。我们认为该激酶在细胞极性转化为极化生长中发挥作用，但在极性本身的建立中不发挥作用。
We hare initiated a study to identify genes regulating cell morphogenesis in the fission yeast Schizosaccharomyces pombe. Five genes have been identified, orb1-orb5, whose mutation gives rise to spherical cells, indicative of an inability to polarize growth. Two further genes have been identified, teal and ban1, whose mutant alleles have disturbed patterns of tip growth, leading to T-shaped and curved cells. In fission yeast, sites of cell wall deposition are defined by actin localization, with actin distributions and therefore growth patterns undergoing cell cycle stage-specific reorganization. Studies of double mutants constructed between orb5-19 and various cdc mutants blocked before and after cell division show that orb5 is required for the re-establishment of polar growth following cytokinesis. This indicates that the mutant allele orb5-19 is defective in the reinitiation of polarized growth, even though actin reorganization to the cell tips occurs normally. orb5 encodes a fission yeast homologue of casein kinase II alpha. We propose that this kinase plays a role in the translation of cell polarity into polarized growth, but not in the establishment of polarity itself.