SPLICING AS A REQUIREMENT FOR BIOGENESIS OF FUNCTIONAL 16S MESSENGER-RNA OF SIMIAN VIRUS-40
SPLICING AS A REQUIREMENT FOR BIOGENESIS OF FUNCTIONAL 16S MESSENGER-RNA OF SIMIAN VIRUS-40
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DOI：
10.1073/pnas.76.9.4317
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
KHOURY, G
中科院分区：
文献类型：
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作者：
GRUSS, P;LAI, CJ;KHOURY, G
SV-40 缺失突变体的构建缺乏特定的晚期病毒 mRNA 的插入序列。构建方法涉及用病毒 mRNA 逆转录的 DNA 片段替换晚期 SV-40 基因的部分。限制性核酸内切酶切割和序列分析证实了突变体 DNA 的精确结构，并证明它们包含[蛋白质] VP1 的遗传信息，包括晚期病毒 RNA 的所有潜在 5'' 末端。因此，该突变体的初级晚期转录产物应具有功能性 16S mRNA 的结构。互补分析和免疫沉淀表明，该突变体中插入序列的删除阻止了 VP1 的表达。这种失败的本质似乎是病毒 RNA 转录后加工的缺陷。剪接显然是某些 mRNA 生物发生中的重要功能。
SV-40 deletion mutants were constructed lacking specifically the intervening sequences for a late viral mRNA. The construction method involved the replacement of portions of the late SV-40 genes with the DNA segment from reverse transcription of the viral mRNA. Restriction endonuclease cleavage and sequence analysis confirmed the precise structure of the mutant DNA and demonstrated that they contained the genetic information for [protein] VP1, including all potential 5'' ends for the late viral RNA. Thus, the primary late transcription product(s) of this mutant should have the structure of functional 16S mRNA. Complementation analysis and immunoprecipitation showed that deletion of the intervening sequences from this mutant prevented the expression of VP1. The nature of this failure appears to be a defect in the posttranscriptional processing of the viral RNA. Splicing is apparently an essential function in the biogenesis of certain mRNA.