Antibody-labeling Techniques
Antibody-labeling Techniques
复制标题
抗体标记技术
DOI：
10.1007/978-3-642-66809-8_2
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
J. Koehler
中科院分区：
文献类型：
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作者：
W. Perkins;J. Koehler
用荧光染料、铁蛋白、辣根过氧化物酶、病毒、血蓝蛋白和放射性碘等可见标记物标记的抗体最近已被广泛用于帮助理解各种生物学现象（Raff，1976; Karnovsky和Unanue，1973;瓦格纳，1973）。例如，在免疫学领域，研究特别集中于定义负责引发细胞和体液免疫应答的淋巴细胞群。为了辨别这些群体，针对每种细胞类型特有的免疫决定簇制备抗体。随后，用适当的可见标记物标记特异性抗体，所述标记物可以用光学显微镜和/或透射电子显微镜（TEM）鉴定。因此，负责细胞免疫应答的细胞（胸腺细胞）被表征为在小鼠中具有抗θ抗体的表面受体（Raff，1969; 1970），并且参与体液免疫应答的细胞（骨髓淋巴细胞）被证明具有抗免疫球蛋白（抗Ig）的受体（Rabellino和Gray，1971; Raff等人，1970; Unanue等人，1971年）。这些和其他淋巴细胞受体的特异性表征和定位目前正在考虑在超微结构水平的许多研究人员。
Antibodies labeled with visual markers such as fluorochromes, ferritin, horseradish peroxidase, viruses, hemocyanin, and radioiodine, have recently been used extensively to help understand a variety of biological phenomena (Raff, 1976; Karnovsky and Unanue, 1973; Wagner, 1973). In the field of immunology, for example, studies have focused specifically on defining lymphoid populations responsible for elicitation of both cellular and humoral immune responses. To discern these populations, antibodies were prepared against immunologic determinants unique to each cell type. Subsequently, the specific antibody was labeled with an appropriate visual marker that could be identified with the light microscope and/or transmission electron microscope (TEM). Cells responsible for cellular immune responses (thymus cells) were thus characterized as possessing surface receptors for anti-theta antibody in mice (Raff, 1969; 1970) and cells involved in humoral immune responses (bone marrow lymphocytes) were shown to possess receptors for anti-immunoglobulin (anti-Ig) (Rabellino and Gray, 1971; Raff et al., 1970; Unanue et al., 1971). Specific characterization and localization of these and other lymphocyte receptors are currently being considered at the ultrastructural level by numerous investigators.