Low-intensity pulsed ultrasound modifies effects of a myelin-related growth inhibitor and enhances neurite re-growth
Low-intensity pulsed ultrasound modifies effects of a myelin-related growth inhibitor and enhances neurite re-growth
复制标题
低强度脉冲超声改变髓磷脂相关生长抑制剂的作用并增强神经突再生
DOI：
10.1109/ultsym.2019.8926300
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Takiguchi Mitsuyoshi
中科院分区：
文献类型：
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作者：
Sasaki Noboru;Kudo Nobuki;Ohta Hiroshi;Takiguchi Mitsuyoshi
轴突再生是脊髓损伤后功能恢复的关键。髓鞘相关的生长抑制物，如Nogo-A，抑制神经突起的再生长和延长。众所周知，低强度脉冲超声(LIPUS)在神经退行性疾病中显示出神经保护作用。在本研究中，我们评估了LIPUS在Nogo-A存在下促进轴突再生的可行性。经Nogo-A处理后，用低强度脉冲超声照射体外培养的大鼠皮质神经元。LIPUS组每细胞突起总长度明显大于非LIPUS组。神经突起数目无明显差异。结果提示，在Nogo-A存在的情况下，LIPUS可促进神经突起的伸长，而对神经突起的萌发和分支无影响。这一初步研究提示LIPUS用于轴突再生的可行性。
Axon regeneration is crucial for the functional recovery after spinal cord injury. Myelin-related growth inhibitors, such as Nogo-A, inhibit neurites regrowth and elongation. Low-intensity pulsed ultrasound (LIPUS) is known to show neuroprotective effects in neurodegenerative diseases. In the present study, we evaluated the feasibility of LIPUS for enhancing axon regeneration in the presence of Nogo-A. Low-intensity pulsed ultrasound was exposed to rat cortical neurons in vitro after Nogo-A treatment. The total length of neurites per cell in LIPUS group was significantly larger than that of non-LIPUS group. There was no significant difference in the number of neurites. The results suggest that LIPUS may enhance the elongation of neurites in the presence of Nogo-A while may not affect on neurites sprouting and branching. This preliminary study implies the feasibility of LIPUS for axonal regeneration.