Fluorometric Determination of Diarrhetic Shellfish Toxins by High-Performance Liquid Chromatography
Fluorometric Determination of Diarrhetic Shellfish Toxins by High-Performance Liquid Chromatography
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DOI：
10.1271/bbb1961.51.877
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发表时间：
1987-03
期刊：
影响因子：
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通讯作者：
Jong-Soo Lee;T. Yanagi;Ritsuko Kenma;T. Yasumoto
中科院分区：
文献类型：
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作者：
Jong-Soo Lee;T. Yanagi;Ritsuko Kenma;T. Yasumoto
将腹泻性贝类中毒的两种主要毒素冈田酸和甲藻毒素-1在甲醇中与9-苯基重氮甲烷进行酯化反应。经SEP-PAK硅胶小柱净化后，在Develosil ODS色谱柱上，以MeCN-MeOH-H20(8：1：1)为流动相进行分析。在365 nm激发波长和412 nm发射波长下，两种毒素衍生物的荧光强度在1~80 ng范围内呈良好的线性关系。
Two principal toxins of diarrhetic shellfish poisoning, okadaic acid and dinophysistoxin-1, were esterified with 9-anthryldiazomethane in methanol. After cleaning with a Sep-pak silica cartridge column, the fluorescent esters were analyzed on a Develosil ODS column with MeCN- MeOH-H20 (8:1:1). The fluorescence intensities of both toxin derivatives measured at an excitation of 365 nm and an emission of 412 nm showed good linearity in the range 1 ~ 80ng.