Intraspecific polyploidy correlates with colonization by arbuscular mycorrhizal fungi in Heuchera cylindrica
Intraspecific polyploidy correlates with colonization by arbuscular mycorrhizal fungi in Heuchera cylindrica
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DOI：
10.1002/ajb2.1294
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发表时间：
2019-06-01
影响因子：
3
通讯作者：
Segraves, Kari A.
中科院分区：
文献类型：
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作者：
Anneberg, Thomas J.;Segraves, Kari A.
众所周知，植物的多倍体会引起植物的生理变化，而这些变化反过来又会影响物种间的相互作用。在多倍体植物中预测的一个主要生理变化是对生长限制性营养素的需求增加。因此，我们预计多倍体会导致对地下共生体的依赖增加，这些共生体提供这些限制生长的营养物质。因此，研究多倍体如何影响植物中的营养互惠关系的重要的第一步是描述二倍体和多倍体与地下互惠者相互作用的速率差异。方法以虎耳草科Heuchera cylindrica为材料，研究多倍体对丛枝菌根真菌（AMF）的影响。我们首次证实了AMF在H.圆柱，然后我们使用现场收集的标本，以量化和比较AMF结构的存在，同时控制特定网站的变化。结果四倍体具有较高的定殖率，总，菌丝，营养交换结构的测量，但是，我们发现，二倍体和四倍体没有不同的泡定殖率。结论多倍体可能改变了植物与地下营养的互惠关系。由于营养交换结构的定植在多倍体中较高，而囊泡定植则不然，因此多倍体可能与AMF伙伴形成更强的关联。控制实验是必要的，以测试这种模式是否是由多倍体AMF定植的直接影响。
Premise Polyploidy is known to cause physiological changes in plants which, in turn, can affect species interactions. One major physiological change predicted in polyploid plants is a heightened demand for growth-limiting nutrients. Consequently, we expect polyploidy to cause an increased reliance on the belowground mutualists that supply these growth-limiting nutrients. An important first step in investigating how polyploidy affects nutritional mutualisms in plants, then, is to characterize differences in the rate at which diploids and polyploids interact with belowground mutualists. Methods We used Heuchera cylindrica (Saxifragaceae) to test how polyploidy influences interactions with arbuscular mycorrhizal fungi (AMF). Here we first confirmed the presence of AMF in H. cylindrica, and then we used field-collected specimens to quantify and compare the presence of AMF structures while controlling for site-specific variation. Results Tetraploids had higher colonization rates as measured by total, hyphal, and nutritional-exchange structures; however, we found that diploids and tetraploids did not differ in vesicle colonization rates. Conclusions The results suggest that polyploidy may alter belowground nutritional mutualisms with plants. Because colonization by nutritional-exchange structures was higher in polyploids but vesicle colonization was not, polyploids might form stronger associations with their AMF partners. Controlled experiments are necessary to test whether this pattern is driven by the direct effect of polyploidy on AMF colonization.