Canine electroencephalographic recording technique: findings in normal and epileptic dogs
Canine electroencephalographic recording technique: findings in normal and epileptic dogs
复制标题
DOI：
10.1016/s1388-2457(03)00347-x
复制
发表时间：
2004-02-01
影响因子：
4.7
通讯作者：
Sica, REP
中科院分区：
文献类型：
--
作者：
Pellegrino, FC;Sica, REP
目的：缺乏标准化的记录技术和正常的参考模式可能阻碍了实验脑电(EEG)在狗身上的应用以及在兽医中的应用。因此，本工作的目的是建立一种记录近头、中头和多头犬种的记录技术，以标准化记录参数，包括鼻脑和前回的电活动，并证明其检测异常活动的可行性。使用三种不同剂量的赛拉津(0.5、1和1.5 mg/kg)来确定哪种药物具有足够的镇静效果以获得无伪影的记录。50只正常狗作为研究对象，9只癫痫犬作为对照。结果：我们找到了脑电记录电极应连接的特定解剖头骨位置；实际上，这些位置对每只狗都是相同的，无论其头骨类型如何。选择剂量为1 mg/kg的赛拉津进行抑制。正常犬以6-12赫兹波为主，皮质各处均可辨认。癫痫犬在临床发作间歇期出现阵发性放电，由尖峰、尖锐和异常的正弦波组成，在临床发作间歇期通常开始于一个区域，后来扩散到整个皮质。结论：与文献报道相比，可以获得更广泛的犬大脑皮质，包括鼻脑和前脑回的电生理覆盖率。肌肉内或皮下注射1毫克/公斤的赛拉津被证明对记录产生了最好的约束。这项技术可以区分癫痫狗的异常活动。(C)2003年国际临床神经生理学联合会。爱思唯尔爱尔兰有限公司出版。版权所有。
Objective: The absence of a standardized recording technique and of normal reference patterns may have hindered the use of experimental electroencephalography (EEG) in dogs and in veterinary medicine as well. Therefore, the aim of the present work was to develop a recording technique for brachycephalic, mesocephalic and dolichocephalic breeds of dogs that would standardize the recording parameters, including the electrical activity of the rhinencephalon and prorean gyrus, and to prove its feasibility for detecting abnormal activity.Methods: We accomplished this task by employing 12 recording electrodes. Three different doses of xylazine (0.5, 1 and 1.5 mg/kg) were used to determine which gave enough sedation to obtain a recording free of artifact. Fifty normal dogs were studied; for comparison 9 epileptic dogs were also explored.Results: We found specific anatomical skull sites where the EEG recording electrodes should be attached; the location of these sites is, in practical terms, the same for every dog, regardless of their skull type. A dose of I mg/kg xylazine was chosen for restraining. Normal dogs displayed predominant 6-12 Hz waves which could be recognized all over the cortex. Epileptic dogs were individualized because of the appearance of paroxysmal discharges, composed of spikes, sharp and anomalous sinusoidal waves which usually started in one area, spreading to the whole cortex later on, in the clinical interictal periods.Conclusions: It was possible to obtain more extensive electrophysiological coverage of the canine cerebral cortex, including the rhinencephalon and prorean gyrus, than previously described in the literature. Intramuscular or subcutaneous xylazine, at a dose of I mg/kg, proved to produce the best restraint for recordings. The technique allowed to distinguish abnormal activity in epileptic dogs. (C) 2003 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.