EFFECTS OF ISCHEMIA ON ULCER WOUND-HEALING - A NEW MODEL IN THE RABBIT EAR
EFFECTS OF ISCHEMIA ON ULCER WOUND-HEALING - A NEW MODEL IN THE RABBIT EAR
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DOI：
10.1097/00000637-199001000-00004
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发表时间：
1990-01-01
影响因子：
1.5
通讯作者：
MUSTOE, TA
中科院分区：
文献类型：
--
作者：
AHN, ST;MUSTOE, TA
为了研究血流改变对兔耳溃疡伤口愈合的影响，设计了两种模型，分别产生最大缺血和最大充血，通过选择性分割三个动脉或静脉中的一个或多个和环形切口，使耳完全存活。从六种变化中选择最佳模型后，通过静脉氧分压、皮肤荧光测定法、脉搏血氧测定法和皮肤温度间接测量组织灌注。在产生6-mm手术溃疡后7天测量伤口愈合。通过静脉氧分压计算的组织氧分压下降到30毫米汞柱，通过第1，3和7天在缺血的耳朵，皮肤温度和血流量测量皮肤荧光法也受到了类似的影响。然而，除了充血耳朵的皮肤温度升高外，没有证据表明组织灌注有显著变化。没有一个缺血性伤口完全上皮化，但一半的对照和充血伤口上皮化。与对照伤口相比，缺血性伤口的肉芽组织形成减少（面积的39%对面积的63%），感染率显著增加（20%对2%），但充血性伤口没有。缺血性溃疡模型是可再现的和可量化的，并且是用于检查药剂以改善血流减少的溃疡愈合的潜在有用模型。充血模型显示血流或伤口愈合没有显著变化。
To study the effects of alteration of blood flow on wound healing in rabbit ear ulcers, two models were designed that produced maximum ischemia and maximum congestion, respectively, with complete survival of the ear by selective division of one or more of three arteries or veins and circumferential incisions. After selection of the best models from six variations, tissue perfusion was measured indirectly by venous oxygen tension, dermofluorometry, pulse oximetry, and skin temperature. Wound healing was measured seven days after creating 6-mm surgical ulcers. The tissue oxygen tension calculated from the venous oxygen tension fell to 30 mm Hg through days 1, 3, and 7 in the ischemic ears, and skin temperature and blood flow measured by dermofluorometry were similarly affected. However, there was no evidence of significant change in tissue perfusion except increased skin temperature in the congested ears. None of the ischemic wounds were epithelialized completely, but half of the control and congested wounds were. The granulation tissue formation was decreased (39% of the area versus 63% of the area) and the infection rate was increased (20% versus 2%) significantly in the ischemic wounds compared with the control wounds but not in the congested wounds. The ischemic ulcer model is reproducible and quantifiable and is potentially a useful model for examining agents to improve ulcer healing where blood flow is decreased. The congestion model showed no significant changes in blood flow or wound healing.