Quantitative analysis of the 4977-bp common deletion of mitochondrial DNA in postmortem frontal cortex from patients with bipolar disorder and schizophrenia
Quantitative analysis of the 4977-bp common deletion of mitochondrial DNA in postmortem frontal cortex from patients with bipolar disorder and schizophrenia
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DOI：
10.1016/j.neulet.2008.05.009
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发表时间：
2008-07-11
影响因子：
2.5
通讯作者：
Kato, Tadafumi
中科院分区：
文献类型：
--
作者：
Fuke, Satoshi;Kametani, Mizue;Kato, Tadafumi
一些报告表明线粒体功能障碍在双相情感障碍和精神分裂症的病理生理学中的作用。我们已经专注于删除线粒体DNA（mtDNA）和双相情感障碍之间的关系。为了研究这种关系，我们开发了一种基于SYBR绿色实时聚合酶链反应的mtDNA常见4977-bp缺失的定量方法。在这项研究中，我们评估了147人（48名对照，49名双相情感障碍患者，50名精神分裂症患者）死后额叶皮层中常见缺失的积累。我们证明了mtDNA常见缺失的年龄依赖性积累（p = 1.09E-10）。女性表现出显着更高的积累的缺失比男性（p = 0.002）。在额叶皮层中，累积和两种主要的精神障碍之间没有显著的联系（p > 0.2）。然而，女性双相情感障碍患者中常见缺失与年龄之间无统计学显著相关性（p = 0.133），双相情感障碍患者中无显著性别差异（p = 0.509）。这些结果表明，年龄和性别的共同缺失的线粒体DNA在前额叶皮层的积累取决于诊断的影响。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Several reports have suggested a role for mitochondrial dysfunction in the pathophysiology of bipolar disorder and schizophrenia. We have focused on the relationship between deleted mitochondrial DNA (mtDNA) and bipolar disorder. To investigate this relationship, we developed a methodology for quantification of the common 4977-bp deletion of mtDNA based on real-time polymerase chain reaction with SYBR Green. In this study, we assessed accumulation of the common deletion in postmortem frontal cortex from 147 individuals (48 controls, 49 patients with bipolar disorder, 50 patients with schizophrenia). We demonstrated age-dependent accumulation of the common deletion of mtDNA (p = 1.09E-10). Females showed significantly higher accumulation of the deletion than did males (p = 0.002). There was no significant association between accumulation and the two studied major mental disorders in the frontal cortex (p > 0.2). However, there was no statistically significant correlation between the common deletion and aging in female patients with bipolar disorder (p = 0.133), and no significant sex difference in patients with bipolar disorder (p = 0.509). These results indicate that aging and sex have effect on accumulation of the common deletion of mtDNA in the prefrontal cortex depending on the diagnosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.