Effects of Helicobacter pylori infection on the link between regenerating gene expression and serum gastrin levels in mongolian gerbils
Effects of Helicobacter pylori infection on the link between regenerating gene expression and serum gastrin levels in mongolian gerbils
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DOI：
10.1097/01.lab.0000106501.56339.ce
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发表时间：
2003-12-01
影响因子：
5
通讯作者：
Genta, RM
中科院分区：
文献类型：
--
作者：
Fukui, H;Franceschi, F;Genta, RM
虽然再生基因（Reg）蛋白被报道对胃上皮细胞具有萎缩作用，但其在人类胃疾病中的参与尚不清楚。我们最近发现，胃泌素和胃粘膜炎症均增强大鼠胃底粘膜中Reg基因的表达。本研究旨在阐明Reg蛋白是否参与幽门螺杆菌诱导的胃炎，以及Reg基因表达是否与血清胃泌素水平有关。用H. pylori菌株。4周后，部分H.用兰索拉唑、阿莫西林和克拉霉素根除幽门螺杆菌感染。Reg基因表达，血清胃泌素水平，胃酸和组织病理学因素的变化的时间过程进行了检查。H.幽门螺杆菌感染后，胃炎开始扩散到胃底粘膜，胃酸开始减少。感染后6周血清胃泌素水平和眼底Reg mRNA表达均显著升高。Reg mRNA在眼底的表达与血清胃泌素水平和胃底粘膜炎症的严重程度显著相关。H.幽门螺杆菌根除，血清胃泌素水平和胃底粘膜炎症恢复正常，Reg mRNA表达的增加被消除。Reg基因与高胃泌素血症和胃底粘膜炎症有关，并可能参与H。幽门引起的胃炎
Although regenerating gene (Reg) protein is reported to have atrophic effect on gastric epithelial cells, its involvement in human gastric diseases is not clear, We have recently shown that both gastrin and gastric mucosal inflammation enhance Reg gene expression in the fundic mucosa in rats. This study was designed to clarify whether Reg protein is involved in Helicobacter pylori-induced gastritis and whether Reg gene expression is linked to serum gastrin levels in this condition. Mongolian gerbils were inoculated with an H. pylori strain isolated from a gastric cancer patient. Four weeks later, some of the gerbils with H. pylori infection were eradicated by lansoprazole, amoxicillin, and clarithromycin. The time courses of changes in Reg gene expression, serum gastrin levels, gastric acidity, and histopathologic factors were examined. Four weeks after H. pylori infection, gastritis started spreading to the fundic mucosa, and gastric acidity started reducing. Serum gastrin levels and Reg mRNA expression in the fundus were significantly increased 6 weeks after infection. Reg mRNA expression in the fundus correlated significantly with both serum gastrin levels and the severity of fundic mucosal inflammation. After H. pylori eradication, serum gastrin levels and fundic mucosal inflammation were normalized, and the increase in Reg mRNA expression was abolished. The Reg gene is associated with hypergastrinemia and fundic mucosal inflammation and may be involved in H. pylori-induced gastritis.