The human ‘T’ genetic region of the HLA linkage group is a polymorphism detected on lectin-activated lymphocytes
The human ‘T’ genetic region of the HLA linkage group is a polymorphism detected on lectin-activated lymphocytes
复制标题
HLA连锁群的人类“T”遗传区域是在凝集素激活的淋巴细胞上检测到的多态性
作者：
E. Gazit;C. Terhorst;E. Yunis
小鼠的主要组织相容性复合体H-2由12个位点组成，分为区域和亚区域1。目前的数据表明，在H-2D的“右侧”有9个基因座聚集在一个区域，即“T区”。这些位点被命名为Qa-1、Qa-2、Qa-3、Qa-4、Qa-5、H-2T、H-31、H-32、TLa（参考文献2）。事实上，“T区”的位点可能是H-2复合体的一部分。这一观点得到了以下事实的支持：TLa、Qa-2和Qa-3位点的基因产物与H-2基因产物具有某些分子特性：具有共同决定分子量为12,000 β2-微球蛋白的双多肽链复合物和分子量为45,000的可变重链（参考文献3）。最近的报道表明，人类可能存在与小鼠“T区”基因产物类似的基因产物。最近从人淋巴母细胞样t细胞系MOLT4中分离出与HLA-A、-B和-C抗原相似但不完全相同的抗原结构（文献4,5）。这些报告提供了β2-微球蛋白或β2-微球蛋白样分子Bt存在的证据，该分子与分子量为MW ~ 45,000的多肽链相关。然而，这些结构是否等同于小鼠的Qa-1、Qa-2或TLa基因产物还有待确定。我们现在报告了鉴定人同种抗血清与细胞表面决定因子反应，在有丝分裂原活化的T淋巴细胞上表达，但在静息T细胞上不表达。采用抗原阻断和调制技术。所鉴定的同种抗原（HT）不同于经典的HLA-A、-B、-C和-DR抗原，但与β2微球蛋白相关。这些决定因素由HLA-A区域的基因编码，可能代表小鼠TLa区域决定因素的人类对应物1 - 3。
The major histocompatibility complex of the mouse, H–2, comprises 12 loci which are grouped into regions and subregions1. Present data indicate that to the ‘right’ of the H–2D there are nine loci clustered into one region, the ‘T region’. These loci are designated Qa-1, Qa-2, Qa-3, Qa-4, Qa-5, H–2T, H–31, H–32, TLa (ref. 2). The loci in the ‘T region’ may, in fact, be part of the H–2 complex. This notion is supported by the fact that gene products of the TLa, Qa-2 and Qa-3 loci share some molecular properties with H–2 products: a two-polypeptide chain complex with a common determinant of molecular weight 12,000 β2-microglobulin and a variable heavy chain of MW 45,000 (ref. 3). Recent reports suggested the possible existence of human analogues to the gene products of the murine ‘T region’. Antigenic structures, similar but not identical with HLA-A, -B and -C antigens, were recently isolated from the human lymphoblastoid T-cell line MOLT4 (refs 4,5). These reports presented evidence for the existence of a β2-microglobulin or a β2-microglobulin-like molecule, Bt, associated with a polypeptide chain of MW∼45,000. However, it remains to be determined whether these structures are equivalent to the mouse Qa-1, Qa-2 or TLa gene products. We now report the identification of human alloantisera that react with cell-surface determinants expressed on mitogen-activated T lymphocytes but not expressed on resting T cells. Antigen blocking and modulation techniques were used. The alloantigens identified (HT) are different from the classical HLA-A, -B, -C and -DR antigens but are associated with β2-microglobulin. These determinants are encoded by genes in the HLA-A region and may represent the human counterpart of the TLa region determinants of the mouse1–3.