Human chromosome 8 linkage map based on short tandem repeat polymorphisms: effect of genotyping errors.
Human chromosome 8 linkage map based on short tandem repeat polymorphisms: effect of genotyping errors.
复制标题
基于短串联重复多态性的人类 8 号染色体连锁图：基因分型错误的影响。
DOI：
10.1016/s0888-7543(05)80297-6
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发表时间：
1992
期刊：
影响因子：
4.4
通讯作者：
Wang,Z
中科院分区：
文献类型：
--
作者：
Tomfohrde,J;Wood,S;Schertzer,M;Wagner,MJ;Wells,DE;Parrish,J;Sadler,LA;Blanton,SH;Daiger,SP;Wang,Z
构建了一个由21个二核苷酸重复序列、1个四核苷酸重复序列和3个RFLP组成的人类8号染色体连锁图谱。该图谱覆盖了从PTER附近到长臂末端q23-q24的大部分染色体长度。雌性图谱的总长度为186 cm，是雄性图谱长度84 cm的两倍多。利用一组含有重排染色体的杂交种对这些多态进行了细胞遗传学定位，这与连锁图谱完全一致。特别努力消除了尽可能多的基因分型错误，包括父母阶段错误。去除误差，与最近的理论预测一致，导致性别平等图谱的总长度减少了10%，从145厘米减少到130厘米。
A linkage map consisting of 21 dinucleotide repeat polymorphisms, 1 tetranucleotide repeat polymorphism, and 3 RFLPs was constructed for human chromosome 8. The map spanned most of the chromosome length from near pter to q23–q24 on the distal portion of the long arm. The total 186 cM length of the female map was over two times the 84 cM length of the male map. Cytogenetic mapping of the polymorphisms using a panel of hybrids containing rearranged chromosomes was completely consistent with the linkage map. Special effort was made to remove as many genotyping errors, including parental phase errors, as possible. Removal of errors, in agreement with recent theoretical predictions, led to reduction of the total length of the sex-equal map by 10% from 145 to 130 cM.