The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators
The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators
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DOI：
10.1105/tpc.15.00567
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发表时间：
2016-02-01
期刊：
影响因子：
11.6
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Xu, Na;Wang, Rongchen;Wang, Yong
我们发现，硝酸盐调节基因2（NRG 2），我们确定了使用正向遗传学，介导的拟南芥硝酸盐信号。NRG 2的突变破坏了硝酸盐处理后的硝酸盐响应基因的诱导铵独立的机制。突变体根中的硝酸盐含量低于野生型，这可能是由于NRT1.1（也称为NPF6.3，编码硝酸盐转运蛋白/受体）的表达减少和NRT1.8（也称为NPF7.2，编码木质部硝酸盐转运蛋白）的上调。遗传和分子数据表明，NRG 2在硝酸盐信号传导中的NRT1.1上游起作用。此外，NRG 2直接与细胞核中的硝酸盐调节剂NLP 7相互作用，但NLP 7响应硝酸盐的核保留不依赖于NRG 2。转录组学分析表明，涉及四个氮相关簇，包括硝酸盐运输和响应硝酸盐的基因在nrg 2突变体中差异表达。一个含氮化合物的运输集群NRT/PTR家族的一些成员的NRG 2和NRT1.1的调节，而没有氮相关的集群显示NRG 2和NLP 7的调节。因此，NRG 2部分通过调节NRT 1.1表达在硝酸盐调节中起关键作用，并且可能通过它们的物理相互作用与NLP 7起作用。
We show that NITRATE REGULATORY GENE2 (NRG2), which we identified using forward genetics, mediates nitrate signaling in Arabidopsis thaliana. A mutation in NRG2 disrupted the induction of nitrate-responsive genes after nitrate treatment by an ammonium-independent mechanism. The nitrate content in roots was lower in the mutants than in the wild type, which may have resulted from reduced expression of NRT1.1 (also called NPF6.3, encoding a nitrate transporter/receptor) and upregulation of NRT1.8 (also called NPF7.2, encoding a xylem nitrate transporter). Genetic and molecular data suggest that NRG2 functions upstream of NRT1.1 in nitrate signaling. Furthermore, NRG2 directly interacts with the nitrate regulator NLP7 in the nucleus, but nuclear retention of NLP7 in response to nitrate is not dependent on NRG2. Transcriptomic analysis revealed that genes involved in four nitrogen-related clusters including nitrate transport and response to nitrate were differentially expressed in the nrg2 mutants. A nitrogen compound transport cluster containing some members of the NRT/PTR family was regulated by both NRG2 and NRT1.1, while no nitrogen-related clusters showed regulation by both NRG2 and NLP7. Thus, NRG2 plays a key role in nitrate regulation in part through modulating NRT1.1 expression and may function with NLP7 via their physical interaction.