Inhibitory effect of activin A on activation of lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells
Inhibitory effect of activin A on activation of lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells
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激活素A对脂多糖刺激的小鼠巨噬细胞RAW264.7细胞活化的抑制作用
DOI：
10.1016/j.cyto.2008.01.010
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发表时间：
2008-04-01
期刊：
影响因子：
3.8
通讯作者：
Liu, Zhong-Hui
中科院分区：
文献类型：
--
作者：
Wang, Shi-Yao;Tai, Gui-Xiang;Liu, Zhong-Hui
激活素A（Activin A）是转化生长因子β（TGF-β）超家族的成员，又称限制素-P（restrictin-P），能抑制LPS激活的小鼠巨噬细胞分泌一氧化氮（NO）和白细胞介素-1 β（IL-1 β）。本研究探讨了激活素A作为一种抗炎因子对脂多糖（LPS）激活的巨噬细胞的调节作用及其可能机制。结果表明，激活素A不仅能抑制LPS诱导的小鼠巨噬细胞系RAW264.7细胞分泌IL-1 β和NO，降低IL-1 β和iNOS的mRNA表达，而且能抑制LPS诱导的细胞胞饮作用。激活素A可明显降低LPS诱导的RAW264.7细胞表面CD68、CD14和Toll样受体4（TLR4）的表达，但对RAW264.7细胞的增殖无明显影响。这些结果表明，激活素A可能通过抑制LPS诱导的巨噬细胞成熟或LPS-TLR 4信号转导，在炎症因子的产生和活化的巨噬细胞的吞噬中发挥重要的下调作用。(c)2008爱思唯尔有限公司保留所有权利。
Activin A is a member of transforming growth factor beta (TGF-beta) superfamily, which is also named restrictin-P, and can inhibit the secretion of nitric oxide (NO) and interleukin-1 beta (IL-1 beta) from LPS-activated mouse macrophages. In this study, the regulation effect and possible mechanism of activin A as an anti-inflammatory factor on lipopolysaccharide (LPS)-activated macrophages were investigated in vitro. It was observed that activin A could not only decrease the secretion of IL-1 beta and NO, as well as the mRNA expressions of IL-1 beta and iNOS, but also suppress the pinocytosis of mouse macrophage cell line RAW264.7 cells induced by LPS. In addition, activin A could obviously reduce the expressions of CD68 and CD14, as well as Toll-like receptor 4 (TLR4) on RAW264.7 cells induced by LPS, but could not influence the proliferation of RAW264.7 cells. These findings suggest that activin A may play an important down-regulation role in inflammatory factor production and phagocytosis of the activated macrophages via suppressing the maturation of LPS-induced macrophages or LPS-TLR4 signal transduction. (c) 2008 Elsevier Ltd. All rights reserved.