Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach
Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach
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DOI：
10.1016/s0968-0004(00)01562-0
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发表时间：
2000-06-01
影响因子：
13.8
通讯作者：
Copley, SD
中科院分区：
文献类型：
--
作者：
Copley, SD
五氯苯酚在鞘氨醇单胞菌中的降解途径可能是在过去几十年中通过从另外两个分解代谢途径中招募酶而演变的。该途径中的第一种和第三种酶，五氯苯酚羟化酶和2，6-二氯氢醌双加氧酶，可能来源于降解天然存在的氯化苯酚途径中的酶。第二种酶是还原性脱卤酶，可能是从通常参与酪氨酸降解的马来酰乙酰乙酸异构酶进化而来的。这显然是最近组装的途径并没有很好地发挥作用：五氯苯酚羟化酶是相当缓慢的，和四氯氢醌脱卤酶受到严重的底物抑制。
The pathway for degradation of the xenobiotic pesticide pentachlorophenol in Sphingomonas chlorophenolica probably evolved in the past few decades by the recruitment of enzymes from two other catabolic pathways. The first and third enzymes in the pathway, pentachlorophenol hydroxylase and 2,6-dichlorohydroquinone dioxygenase, may have originated from enzymes in a pathway for degradation of a naturally occurring chlorinated phenol. The second enzyme, a reductive dehalogenase, may have evolved from a maleylacetoacetate isomerase normally involved in degradation of tyrosine. This apparently recently assembled pathway does not function very well: pentachlorophenol hydroxylase is quite slow, and tetrachlorohydroquinone dehalogenase is subject to severe substrate inhibition.