C-fos expression in spinal cord and brainstem following noxious stimulation and electroacupuncture plus noxious stimulation.
C-fos expression in spinal cord and brainstem following noxious stimulation and electroacupuncture plus noxious stimulation.
复制标题
伤害性刺激和电针加伤害性刺激后脊髓和脑干中 C-fos 的表达。
DOI：
10.3727/036012995816357087
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发表时间：
1995
影响因子：
0.3
通讯作者：
X. Cao
中科院分区：
文献类型：
--
作者：
H. Wang;K. Y. Li;G. Wu;X. Cao
本研究采用免疫组织化学方法检测伤害性刺激及针刺加伤害性刺激后脊髓和脑干c-fos样蛋白的表达，以追踪伤害性刺激后脊髓和脑干神经通路的变化。结果表明，在伤害性刺激组和针刺足三里、兰卫加伤害性刺激组中，腰骶髓后角（I、II、V、X层）、脊髓背角（N.外侧网状神经，侧旁巨细胞网状核，A1区，n.孤束核中缝大核、蓝斑核、神经背缝和导水管周围灰质。对照组上述结构未见明显c-fos表达。在腰骶段脊髓，针刺加伤害性刺激组FLI核团的数目多于伤害性刺激组，而在蓝斑和中脑导水管周围灰质腹侧部，针刺加伤害性刺激组FLI核团的数目少于伤害性刺激组。提示针刺和伤害性刺激可能通过脊髓背角激活内源性痛觉调制系统，从而抑制伤害性刺激引起的蓝斑和中脑导水管周围灰质腹侧部c-fos表达。
The present study was to trace the neuronal pathway in the spinal cord and brainstem following noxious stimulation and acupuncture plus noxious stimulation by using immunohistochemical technique to detect the expression of c-fos like protein. The results showed that in both the noxious stimulation group and acupuncture of Zusanli(St.36) and Lanwei(Extra 33) points plus noxious stimulation group a lot of Fos-like immunoreactive (FLI) cell nuclei appeared in the following structures: dorsal horn of lumbar and sacral spinal cord (Laminae I, II, V and X), n. reticularis lateralis, n. reticularis paragigantocellularis lateralis, area A1, n. tractus solitarii, n. raphe magnus, locus coeruleus, n. raphe dorsalis and the periaqueductal grey. In the control group no obvious c-fos expression was shown in the structures mentioned above. It was also shown that in the lumbar and sacral spinal cord the number of FLI nuclei in the acupuncture plus noxious stimulation group was more than that in the noxious stimulation group, but in locus coeruleus and the ventral part of the periaqueductal grey the number of FLI nuclei in the acupuncture plus noxious stimulation group was less than that of the noxious stimulation group. The results imply that acupuncture and noxious stimulation may activate the intrinsic pain modulating system through the spinal dorsal horn, then suppress the c-fos expression induced by noxious stimulation in the locus coeruleus and ventral part of the periaqueductal grey.