A Highly Sensitive Glucose Biosensor Based on Gold Nanoparticles/Bovine Serum Albumin/Fe3O4 Biocomposite Nanoparticles
A Highly Sensitive Glucose Biosensor Based on Gold Nanoparticles/Bovine Serum Albumin/Fe3O4 Biocomposite Nanoparticles
复制标题
基于金纳米粒子/牛血清白蛋白/Fe3O4生物复合纳米粒子的高灵敏葡萄糖生物传感器
DOI：
10.1016/j.electacta.2016.11.162
复制
发表时间：
2016-12-20
影响因子：
6.6
通讯作者：
Liu, Jianhong
中科院分区：
文献类型：
--
作者：
He, Chuanxin;Xie, Minsui;Liu, Jianhong
This study presents the synthesis and characterization of a novel glucose electrochemical biosensor based on gold nanoparticles/bovine serum albumin/Fe3O4 (AuNPs/BSA/Fe3O4) composite nanoparticles. The prepared magnetic composite nanoparticles consist of a BSA protein shell, providing biocompatible environment for enzyme immobilization. Furthermore, in situ immobilization of gold nanoparticles greatly enhances the performance of electron transfer. Using these composite nanoparticles, the model enzyme, glucose oxidase (GOx) was readily adsorbed to surface so as the GOx/AuNPs/BSA/Fe3O4 electrochemical biosensor was developed. The electrochemical performance of the biosensor was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry. Under the optimal preparation conditions, the resultant biosensor has a short response time of 0.8 s, an excellent sensitivity of 115.3 mu A mM(-1) cm(-2), and a linear dynamic range from 0.25 to 7.0 mM with a low detection limit of 3.54 mu M (S/N = 3). The high sensitivity, good reproducibility and stability of the biosensor presented in this study show that the as-prepared AuNPs/BSA/Fe3O4 composite nanoparticles are a promising material for the development and construction of biosensors. (C) 2016 Elsevier Ltd. All rights reserved.
