Silencing of Target Chitinase Genes via Oral Delivery of dsRNA Caused Lethal Phenotypic Effects in Mythimna separata (Lepidoptera: Noctuidae)
Silencing of Target Chitinase Genes via Oral Delivery of dsRNA Caused Lethal Phenotypic Effects in Mythimna separata (Lepidoptera: Noctuidae)
复制标题
通过口服 dsRNA 沉默目标几丁质酶基因可导致粘虫（鳞翅目：夜蛾科）致死表型效应
DOI：
10.1007/s12010-016-2254-x
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发表时间：
2017-02-01
影响因子：
3
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
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作者：
Cao, Budao;Bao, Wenhua;Wuriyanghan, Hada
夜蛾（鳞翅目：夜蛾科）是一种多食、迁徙的玉米害虫。近年来，稻瘟病的爆发对中国玉米生产造成了严重的危害。RNAi （RNA干扰）是一种基因沉默技术，在模式生物和非模式生物中都有应用，特别是在后者无法获得反向遗传研究工具的情况下。RNAi方法在许多植物病原菌中得到了广泛的研究，并被用于产生抗害虫转基因植物。我们建议利用这一技术来沉默白僵菌的内源基因，从而为这种昆虫提供一种生物防治方法。饲喂几丁质酶靶基因的dsRNAs可显著降低其转录水平。此外，几丁质酶靶基因的沉默导致表型效应，如体重减轻和死亡率增加。本研究为该昆虫提供了逆向遗传研究工具和潜在的防治策略。
Mythimna separata walker (Lepidoptera: Noctuidae) is a polyphagous, migratory corn pest. Outbreak of M. separata has led to severe damage to corn production recently in China. RNAi (RNA interference) is a gene silencing technology applied both in model and non-model organisms, and it is especially useful for the latter in which the reverse genetic research tools are not available. RNAi approach was broadly investigated in many plant pathogens and was used for the generation of anti-pest transgenic plants. We are proposing to use this technology to silence M. separata endogenous genes, thereby, providing a biocontrol method for this insect. Feeding of dsRNAs for target Chitinase genes resulted in substantial decreases of their transcript levels in M. separata. Furthermore, silencing of target Chitinase genes led to phenotypic effects such as reduced body weight and increased mortality. Our study provided both reverse genetic research tool and potential control strategy for this insect species.