Lineage restriction maintains a stable organizer cell population at the zebrafish midbrain-hindbrain boundary
Lineage restriction maintains a stable organizer cell population at the zebrafish midbrain-hindbrain boundary
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DOI：
10.1242/dev.01862
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发表时间：
2005-07-01
期刊：
影响因子：
4.6
通讯作者：
Brand, M
中科院分区：
文献类型：
--
作者：
Langenberg, T;Brand, M
脊椎动物的后脑被细分为多个部分，称为神经节，它们是基因表达、细胞分化和行为的单位。此类片段的一个关键特性是细胞显示出跨片段边界混合的受限能力 - 称为谱系限制。为了解决中脑-后脑边界 (mhb) 区域的分割问题，我们通过获取转基因斑马鱼系中发育中的 mhb 区域的延时影片来分析活胚胎中的单细胞行为。我们追踪了数百个细胞核的运动，并通过将它们的位置与中脑标记的表达相匹配，我们证明中脑和后脑细胞来自两个不同的细胞群。单细胞标记和对其后代分布的分析表明，谱系限制可能是在原肠胚形成晚期建立的。我们的研究结果表明，分割作为早期大脑发育的组织原则可以扩展到 mhb 区域。我们认为谱系限制有助于限制 mhb 组织者细胞群的位置。
The vertebrate hindbrain is subdivided into segments, termed neuromeres, that are units of gene expression, cell differentiation and behavior. A key property of such segments is that cells show a restricted ability to mix across segment borders - termed lineage restriction. In order to address segmentation in the midbrain-hindbrain boundary (mhb) region, we have analyzed single cell behavior in the living embryo by acquiring time-lapse movies of the developing mhb region in a transgenic zebrafish line. We traced the movement of hundreds of nuclei, and by matching their position with the expression of a midbrain marker, we demonstrate that midbrain and hindbrain cells arise from two distinct cell populations. Single cell labeling and analysis of the distribution of their progeny shows that lineage restriction is probably established during late gastrulation stages. Our findings suggest that segmentation as an organizing principle in early brain development can be extended to the mhb region. We argue that lineage restriction serves to constrain the position of the mhb organizer cell population.