Quantitative measurement of melanin as tyrosine equivalents and as weight of purified melanin.
Quantitative measurement of melanin as tyrosine equivalents and as weight of purified melanin.
复制标题
以酪氨酸当量和纯化黑色素重量的形式定量测量黑色素。
DOI：
--
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
M. Nakayasu
中科院分区：
文献类型：
--
作者：
A. Oikawa;M. Nakayasu
以前我们报道了小鼠黑色素瘤细胞培养物中黑色素合成的化学计量研究（1）。在该系统中，在黑色素合成的Raper-Mason-Nicolaus途径中没有中间体的积累，并且羟基化酪氨酸的量与在黑色素合成中涉及的一系列反应期间从酪氨酸的苯环的3位和5位以水形式释放的氢定量相关。因此，新合成的黑色素的量可以表示为酪氨酸当量。本文介绍了一种以无蛋白黑色素为标准比色测定黑色素的方法，并与酪氨酸当量法进行了比较。对于这种方法，开发了一种新的程序来溶解黑色素。
Previously we reported stoichiometric studies on melanin synthesis in cultures of mouse melanoma cells (1). In this system there was no accumulation of intermediates in the Raper-Mason-Nicolaus pathway for melanin synthesis and the amount of hydroxylated tyrosine was quantitatively related to the release of hydrogen as water from positions 3 and 5 of the benzene ring of tyrosine during the series of reactions involved in melanin synthesis. Therefore, the amount of newly synthesized melanin could be expressed as tyrosine equivalents. The present paper describes a colorimetric method for determination of melanin, using protein-free melanin as standard, and values are compared with those expressed as tyrosine equivalents. For this method, a new procedure was developed to solubilize melanin.