Infrared spectroscopy for neurochemical monitoring of alcohol and its metabolites.
Infrared spectroscopy for neurochemical monitoring of alcohol and its metabolites.
复制标题
用于酒精及其代谢物神经化学监测的红外光谱。
DOI：
10.1117/12.2651704
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hutter,Tanya
中科院分区：
文献类型：
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作者：
Lee,Tse-Ang;Peng,Jessie;Kowal,Gina;Gonzales,Rueben;Hutter,Tanya
连续测量乙醇及其代谢产物乙醛和乙酸的浓度对酒精研究界非常重要。大多数研究仅测量乙醇，因为准确测量所有三种化合物具有挑战性。使用微透析技术进行组织/脑内的测量，然后使用气相色谱法（GC）进行离线分析。为了实现乙醇及其代谢产物的同时测定，人们可以利用红外（IR）光谱作为一种快速和无试剂的方法。在这里，我们报告的可行性研究，使用红外光谱同时测量乙醇，乙醛和乙酸在水溶液中。在不同的光路长度和使用衰减全反射（ATR）的透射模式中的不同浓度进行了测量。对三种化合物的混合物进行体外微透析，并使用IR测量收集的样品，以证明同时定量三种分析物浓度的能力。最后，为了克服微透析技术的局限性，使用渐逝场红外光谱直接测量可以是一种潜在的替代方案。一种疏水聚合物涂层，吸附乙醇和排斥水，可以提高灵敏度。测量了ATR晶体表面聚甲基丙烯酸甲酯（PMMA）和聚二甲基硅氧烷（PDMS）涂层的吸附动力学。这两种聚合物表现出优先吸附乙醇超过水。
Continuous measurement of the concentrations of ethanol and its metabolites, acetaldehyde and acetic acid,in vivois important for the alcohol research community. Most studies only measure ethanol because accurate measurement of all three compounds is challenging. Measurement inside tissue/brain is done using a microdialysis technique, followed by off-line analysis using gas chromatography (GC). To realize simultaneous measurement of ethanol and its metabolites, one can take advantage of infrared (IR) spectroscopy as a rapid and reagent-free method. Here we report a feasibility study of using IR spectroscopy to simultaneously measure ethanol, acetaldehyde and acetic acid in aqueous solution. Different concentrations in transmission mode at different optical pathlengths and using attenuated total reflectance (ATR) were measured. In vitro microdialysis was performed on the mixture of the three compounds, and the collected sample was measured using IR to demonstrate the capability of quantifying the concentrations of the three analytes simultaneously. Lastly, to overcome the limitations of the microdialysis technique, direct measurement using evanescent-field IR spectroscopy can be a potential alternative. A hydrophobic polymer coating that adsorbs ethanol and excludes water, could improve sensitivity. Sorption kinetics in polymethyl methacrylate (PMMA) and polydimethylsiloxane (PDMS) coatings on an ATR crystal were measured. Both polymers demonstrate preferential adsorption of ethanol over water.