Chloroplast Division and Differentially Regulated Expression of FtsZ1 and FtsZ2 in the Synchronous Culture of Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae)
Chloroplast Division and Differentially Regulated Expression of FtsZ1 and FtsZ2 in the Synchronous Culture of Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae)
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DOI：
10.1508/cytologia.77.59
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发表时间：
2012-03
期刊：
影响因子：
1
通讯作者：
Nobuko Sumiya;T. Yamazaki;Satomi Owari;Maki Yamamoto;Koichi Watanabe;S. Kawano
中科院分区：
文献类型：
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作者：
Nobuko Sumiya;T. Yamazaki;Satomi Owari;Maki Yamamoto;Koichi Watanabe;S. Kawano
在植物中，原核生物叶绿体分裂蛋白 FtsZ 分为 FtsZ1 和 FtsZ2 家族。为了阐明单细胞绿藻微绿藻中 FtsZ1 和 FtsZ2 表达与叶绿体分裂之间的关系，我们比较了在 2 种营养不同的培养基中培养的藻类中的 FtsZ1 和 FtsZ2 表达。在丰富培养基中生长的细胞在整个细胞分裂周期中具有 1 个 FtsZ 环，而在无机培养基中的细胞在叶绿体分裂阶段仅显示 1 个 FtsZ 环。丰富培养基中的 FtsZ1 和 FtsZ2 mRNA 水平均高于无机培养基中的水平。然而，在富集培养基和无机培养基中培养的细胞之间的转录物数量在 FtsZ2 中观察到的差异比在 FtsZ1 中大得多，这表明 FtsZ 基因之间存在不同的基因调控机制。在无机培养基中培养的芽孢杆菌细胞表现出细胞分裂光周期，而在有机培养基中培养的细胞在光/暗循环下持续增殖。尽管 FtsZ1 在丰富培养基中的整个叶绿体分裂周期中持续表达，但在无机培养基中的光/暗循环下，FtsZ1 mRNA 水平的增加与叶绿体分裂同时进行。 FtsZ2 在两种媒体中均不断表达。讨论了通过调节 FtsZ 环形成来控制叶绿体分裂。
In plants, the prokaryote-derived chloroplast division protein FtsZ is divided into the FtsZ1 and FtsZ2 families. To clarify the relationship between FtsZ1 and FtsZ2 expression and chloroplast division in the unicellular green alga Nannochloris bacillaris, we compared FtsZ1 and FtsZ2 expression in alga cultured in 2 nutritionally different media. Cells grown in a rich medium had 1 FtsZ ring throughout the cell division cycle, whereas cells in an inorganic medium showed only 1 FtsZ ring during the chloroplast division phase. Both FtsZ1 and FtsZ2 mRNA levels in the rich medium were higher than those in the inorganic medium. However, a much larger difference in the amount of transcripts between cells cultured in the rich and in the inorganic media was observed in FtsZ2 than that in FtsZ1, suggesting different gene regulatory mechanisms between the FtsZ genes. N. bacillaris cells cultured in inorganic medium showed cell division photoperiodicity, whereas cells cultured in organic media proliferated continuously under a light/dark cycle. Although FtsZ1 was expressed constantly throughout the chloroplast division cycle in the rich medium, the increase in FtsZ1 mRNA level was simultaneous with chloroplast division under a light/dark cycle in the inorganic medium. FtsZ2 was expressed constantly in both media. The control of chloroplast division by regulating FtsZ ring formation is discussed.