Latex bead-based artificial antigen-presenting cells induce tumor-specific CTL responses in the native T-cell repertoires and inhibit tumor growth
Latex bead-based artificial antigen-presenting cells induce tumor-specific CTL responses in the native T-cell repertoires and inhibit tumor growth
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基于乳胶珠的人工抗原呈递细胞在天然 T 细胞库中诱导肿瘤特异性 CTL 反应并抑制肿瘤生长
DOI：
10.1016/j.imlet.2013.01.003
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发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Zhang, Jianqiong
中科院分区：
文献类型：
--
作者：
Shen, Chuanlai;Cheng, Kai;Zhang, Jianqiong
将H-2K(B)/Trp2四聚体与抗CD28和抗4-1BB抗体偶联到细胞大小的乳胶珠上，经静脉和皮下注射到初生鼠和抗原致敏的小鼠(B6，H-2K(B))体内，形成无细胞人工抗原提呈细胞(AAPC)。通过检测细胞表面CD69和CD25、细胞内干扰素-γ四聚体染色和黑色素瘤细胞的杀瘤活性来评价各模型小鼠天然T细胞系中的肿瘤抗原特异性CTL反应。此外，aAPC有效地抑制了皮下肿瘤的生长，并显著延缓了荷瘤小鼠的肿瘤进展。这些数据表明，基于珠粒的aAPC代表了癌症或持续性感染的主动免疫治疗的潜在策略。(C)2013爱思唯尔B.V.保留所有权利。
Cell-free artificial antigen-presenting cells (aAPCs) were generated by coupling H-2K(b)/TRP2 tetramers together with anti-CD28 and anti-4-1BB antibodies onto cell-sized latex beads and injected,intravenously and subcutaneously into nave mice and antigen-primed mice (B6, H-2K(b)). Vigorous tumor antigen-specific CTL responses in the native T-cell repertoire in each mouse model were elicited as evaluated by measuring surface CD69 and CD25, intracellular IFN-gamma tetramer staining and cytolysis of melanoma cells. Furthermore, the aAPCs efficiently inhibited subcutaneous tumor growth and markedly delayed tumor progression in tumor-bearing mice. These data suggest that bead-based aAPCs represent a potential strategy for the active immunotherapy of cancers or persistent infections. (c) 2013 Elsevier B.V. All rights reserved.