Sex-dependent differences in the effects of aging on antioxidant defense mechanisms of rat liver.
Sex-dependent differences in the effects of aging on antioxidant defense mechanisms of rat liver.
复制标题
衰老对大鼠肝脏抗氧化防御机制影响的性别依赖性差异。
DOI：
10.1016/0304-4165(91)90061-k
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Snowden,CD
中科院分区：
文献类型：
--
作者：
Rikans,LE;Moore,DR;Snowden,CD
有关影响肝毒性损伤敏感性的年龄相关因素的信息对老年医学和环境健康很重要。本研究的目的是确定年龄相关的变化是否发生在肝脏的抗氧化防御机制的雄性和雌性Fischer 344大鼠。肝匀浆和线粒体后上清液馏分从大鼠年龄为4，14，24和29个月进行了分析，抗氧化酶活性和维生素E和丙二醛含量。过氧化氢酶和谷胱甘肽还原酶活性的变化，可以被描述为性别决定的差异，在老年消失。胞浆超氧化物歧化酶和谷胱甘肽过氧化物酶的活性表现出性别依赖的活性变化，但不受老化。雄性大鼠的肝脏维生素E浓度低于雌性大鼠，并随年龄增加而增加。肝脏丙二醛浓度也低于男性比女性，丙二醛含量增加，老年男性和老年女性减少。结果表明，大鼠肝脏酶和非酶抗氧化防御机制的年龄相关变化是性别依赖性的。此外，与其他大鼠研究结果的比较表明，衰老的影响也可能取决于大鼠的品系。
Information about age-related factors that influence sensitivity to hepatotoxic injury is important to geriatric medicine and environmental health. The purpose of the present study was to determine whether age-associated changes occur in hepatic antioxidant defense mechanisms of male and female Fischer 344 rats. Liver homogenates and post-mitochondrial supernatant fractions from rats aged 4, 14, 24 and 29 months were analyzed for antioxidant enzyme activities and for vitamin E and malondialdehyde content. Age-associated changes in catalase and glutathione reductase activities were observed that could be described as sex-determined differences that disappeared in old age. Cytosolic superoxide dismutase and glutathione peroxidase activities displayed sex-dependent variations in activity but were unaffected by aging. Hepatic vitamin E concentrations were lower in male rats than in female rats and increased with age. Hepatic malondialdehyde concentrations also were lower in males than in females; malondialdehyde content increased in old males and decreased in old females. The results indicate that age-associated changes in enzymatic and nonenzymatic antioxidant defense mechanisms of rat liver are sex-dependent. In addition, comparison with findings from other studies in rats suggests that the effects of aging may also depend on the strain of rat.