RNA stem structure governs coupling of dicing and gene silencing in RNA interference.
RNA stem structure governs coupling of dicing and gene silencing in RNA interference.
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DOI：
10.1073/pnas.1710298114
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发表时间：
2017-11-28
影响因子：
11.1
通讯作者：
Myong S
中科院分区：
文献类型：
--
作者：
Koh HR;Ghanbariniaki A;Myong S
RNAi is an RNA-induced gene-silencing pathway that is shared among various organisms. Better understanding of RNAi is urgently needed to improve our knowledge of RNA-mediated gene regulation and to advance the field of functional genomics and its application to gene therapy. We counted with high precision the number of transcripts in each cell’s nucleus and cytoplasm as a function of silencing time to investigate the role of small RNA secondary structures such as loop length and stem mismatches. We screened various structural features of small RNAs and discovered a distinct role of each structural element that contributes to gene-silencing kinetics. We provide a helpful guideline for designing small RNAs for more efficient gene silencing. PremicroRNAs (premiRNAs) possess secondary structures consisting of a loop and a stem with multiple mismatches. Despite the well-characterized RNAi pathway, how the structural features of premiRNA contribute to dicing and subsequent gene-silencing efficiency remains unclear. Using single-molecule FISH, we demonstrate that cytoplasmic mRNA, but not nuclear mRNA, is reduced during RNAi. The dicing rate and silencing efficiency both increase in a correlated manner as a function of the loop length. In contrast, mismatches in the stem drastically diminish the silencing efficiency without impacting the dicing rate. We show that this decoupling effect is not due to the loading to the RNA-induced silencing complex, RNA uptake, or cellular dicing. We postulate that the stem mismatches perturb the handover of the cleaved miRNAs from Dicer to Argonaute, leading to poor strand selection. Our results imply that the stem structures prevalent in cellular miRNAs have suboptimal silencing efficiency.
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DOI：
10.1038/nrg3355
发表时间：
2013-02
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
16.8
作者：
Haley, B;Zamore, PD
通讯作者：
Zamore, PD
影响因子：
56.9
作者：
Femino, A;Fay, FS;Singer, RH
通讯作者：
Singer, RH
影响因子：
21.3
作者：
Lee, Young Sik;Pressman, Sigal;Andress, Arlise P.;Kim, Kevin;White, Jamie L.;Cassidy, Justin J.;Li, Xin;Lubell, Kim;Lim, Do Hwan;Cho, Ik Sang;Nakahara, Kenji;Preall, Jonathan B.;Bellare, Priya;Sontheimer, Erik J.;Carthew, Richard W.
通讯作者：
Carthew, Richard W.
影响因子：
46.9
作者：
Kim, DH;Behlke, MA;Rossi, JJ
通讯作者：
Rossi, JJ