Aquaporin1 regulates development, secondary metabolism and stress responses in Fusarium graminearum
Aquaporin1 regulates development, secondary metabolism and stress responses in Fusarium graminearum
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DOI：
10.1007/s00294-018-0818-8
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发表时间：
2018-10-01
期刊：
影响因子：
2.5
通讯作者：
Yu, Jinfeng
中科院分区：
文献类型：
--
作者：
Ding, Mingyu;Li, Jing;Yu, Jinfeng
子囊菌纲真菌禾谷镰孢（Fusarium graminearum）是小麦和大麦赤霉病的病原体，已成为真菌植物病原体研究的主要模式生物。水通道蛋白（AQP）参与了酵母、植物和动物体内水、甘油和其他多种小分子物质的转运。然而，这些蛋白质在植物病原真菌中的作用还不清楚。在此，我们鉴定并试图阐明这五个水通道蛋白基因在F.禾谷早熟禾系统发育分析表明，FgAQP可分为两个分支，FgAQP 1位于第一分支。AQP 1突变体形成白色菌落，气生菌丝较长，分生孢子和囊壳形成减少。AQP 1突变分生孢子形态异常，萌发异常。AQP 1突变体和野生型菌株的致病性相同，而突变体产生的脱氧雪腐镰刀菌烯醇（DON）的数量显着增加。AQP 1突变体也表现出增加的抗渗透压和氧化应激以及细胞壁扰动剂。利用FgAQP 1-GFP和DAPI染色，我们发现FgAQP 1定位于分生孢子的核膜。重要的是，FgAQP 1的缺失增加了分生孢子自噬的严重性。综上所述，这些结果表明FgAQP 1参与了菌丝发育、胁迫反应、次生代谢以及有性和无性生殖。禾谷早熟禾与AQP 1突变体不同，AQP 2、AQP 3、AQP 4和AQP 5突变体没有可变的表型。
The Ascomycete fungus Fusarium graminearum, the causal agent of Fusarium head blight of wheat and barley, has become a predominant model organism for the study of fungal phytopathogens. Aquaporins (AQPs) have been implicated in the transport of water, glycerol, and a variety of other small molecules in yeast, plants and animals. However, the role of these proteins in phytopathogenic fungi is not well understood. Here, we identified and attempted to elucidate the function of the five aquaporin genes in F. graminearum. The phylogenetic analysis revealed that FgAQPs are divided into two clades, with FgAQP1 in the first clade. The AQP1 mutant formed whitish colonies with longer aerial hyphae and reduced conidiation and perithecium formation. The AQP1 mutant conidia were morphologically abnormal and appeared to undergo abnormal germination. The AQP1 mutant and the wild type strain were equally pathogenic, while the mutant produced significantly higher quantities of deoxynivalenol (DON). The AQP1 mutant also exhibited increased resistance to osmotic and oxidative stress as well as cell-wall perturbing agents. Using FgAQP1-GFP and DAPI staining, we found that FgAQP1 is localized to the nuclear membrane in conidia. Importantly, deletion of FgAQP1 increased the severity of conidium autophagy. Taken together, these results suggest that FgAQP1 is involved in hyphal development, stress responses, secondary metabolism, and sexual and asexual reproduction in F. graminearum. Unlike the AQP1 mutant, the AQP2, AQP3, AQP4 and AQP5 mutants had no variable phenotypes.