Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures
Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures
复制标题
DOI：
10.1038/ng.2007.49
复制
发表时间：
2008-01-01
期刊：
影响因子：
30.8
通讯作者：
Oliver, Stephen G.
中科院分区：
文献类型：
--
作者：
Delneri, Daniela;Hoyle, David C.;Oliver, Stephen G.
在不同营养环境（葡萄糖限制，铵限制，磷酸盐限制和白色葡萄汁）中生长的连续培养物中使用竞争实验，我们鉴定了显示单倍不足表型（半合子时生长速率降低）或单倍熟练表型（半合子时生长速率增加）的基因。单倍型基因（在葡萄糖限制、铵限制、磷酸盐限制和白色葡萄汁环境中分别为815、1、194、733和654）经常在特定的环境背景下显示出该表型。例如，编码泛素化途径或蛋白酶体组分的基因在氮限制条件下表现出单倍能力，其中蛋白质保守可能是有益的。单倍不足更可能在所有环境中观察到，就像决定细胞极性生长的基因一样。单倍型基因似乎随机分布在基因组中，而单倍型不足的基因（685，765，1,277和217在葡萄糖限制，铵限制，磷酸盐限制和白色葡萄汁环境中，分别）在染色体III上过度代表。这条染色体决定了酵母的交配类型，而单倍不足基因的集中可能是防止其丢失的机制。
Using competition experiments in continuous cultures grown in different nutrient environments (glucose limited, ammonium limited, phosphate limited and white grape juice), we identified genes that show haploinsufficiency phenotypes (reduced growth rate when hemizygous) or haploproficiency phenotypes (increased growth rate when hemizygous). Haploproficient genes (815, 1,194, 733 and 654 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) frequently show that phenotype in a specific environmental context. For instance, genes encoding components of the ubiquitination pathway or the proteasome show haploproficiency in nitrogen-limited conditions where protein conservation may be beneficial. Haploinsufficiency is more likely to be observed in all environments, as is the case with genes determining polar growth of the cell. Haploproficient genes seem randomly distributed in the genome, whereas haploinsufficient genes (685, 765, 1,277 and 217 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) are over-represented on chromosome III. This chromosome determines a yeast's mating type, and the concentration of haploinsufficient genes there may be a mechanism to prevent its loss.