High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
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高密度 SNP 图谱有利于 QTL 精细定位和花生抗黄曲霉候选基因的发现
DOI：
10.1007/s00122-020-03594-0
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发表时间：
2020-04-13
影响因子：
5.4
通讯作者：
Zhuang, Weijian
中科院分区：
文献类型：
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作者：
Khan, Shahid Ali;Chen, Hua;Zhuang, Weijian
利用SLAF-seq定位了两个新的花生抗黄曲霉QTL并鉴定了候选基因。花生中黄曲霉毒素污染是世界范围内危害人类健康的严重食品安全问题。宿主植物对真菌感染的抗性和黄曲霉毒素的减少对于减轻这一问题至关重要。这些抗性连锁标记的鉴定可用于分子标记辅助育种。在这里，我们报告了两个高密度遗传连锁图谱的构建1975个SNP位点和5022个SNP位点，分别。结果表明，在A03和B 04染色体上分别定位到两个一致的数量性状位点qRAF-3-1和qRAF-14-1。QTL qRAF-3-1定位在1.67 cM范围内，解释的表型方差（PVE）大于19%，而qRAF-14-1定位在1.34 cM范围内，解释的表型方差（PVE）为5.15%。与参考基因组相比，定位的QTL qRAF-3-1和qRAF-14-1分别位于1.44 Mbp和2.22 Mbp的物理距离内，分别含有67和137个基因。在已鉴定的候选基因中，有6个基因在两个QTL区域内具有相同的功能。此外，还鉴定了抗病基因RPP 13-like protein 1（RPP 13）、脂氧合酶（Lox）、WRKY转录因子（WRKY）和细胞色素P450 71 B34。使用微阵列分析，基因对A。RPP 13基因、含五肽重复序列的蛋白基因和Lox基因可能是黄曲霉抗性的候选基因。黄的这些QTL和候选基因将进一步促进标记的开发和基因的验证，以用于抗A.花生中的黄色
Two novel resistant QTLs mapped and candidate genes identified for Aspergillus flavus resistance in cultivated peanut using SLAF-seq. Aflatoxin contamination in peanuts caused by Aspergillus flavus is a serious food safety issue for human health around the world. Host plant resistance to fungal infection and reduction in aflatoxin are crucial for mitigating this problem. Identification of the resistance-linked markers can be used in marker-assisted breeding for varietal development. Here we report construction of two high-density genetic linkage maps with 1975 SNP loci and 5022 SNP loci, respectively. Two consistent quantitative trait loci (QTL) were identified as qRAF-3-1 and qRAF-14-1, which located on chromosomes A03 and B04, respectively. QTL qRAF-3-1 was mapped within 1.67 cM and had more than 19% phenotypic variance explained (PVE), while qRAF-14-1 was located within 1.34 cM with 5.15% PVE. While comparing with the reference genome, the mapped QTLs, qRAF-3-1 and qRAF-14-1, were located within a physical distance of 1.44 Megabase pair (Mbp) and 2.22 Mbp, harboring 67 and 137 genes, respectively. Among the identified candidate genes, six genes with the same function were found within both QTLs regions. In addition, putative disease resistance RPP13-like protein 1 (RPP13), lipoxygenase (Lox), WRKY transcription factor (WRKY) and cytochrome P450 71B34 genes were also identified. Using microarray analysis, genes responded to A. flavus infection included coding for RPP13, pentatricopeptide repeat-containing-like protein, and Lox which may be possible candidate genes for resistance to A. flavus. The QTLs and candidate genes will further facilitate marker development and validation of genes for deployment in the molecular breeding programs against A. flavus in peanuts.