Steryl glucoside is a lipid mediator in stress-responsive signal transduction
Steryl glucoside is a lipid mediator in stress-responsive signal transduction
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DOI：
10.1247/csf.27.157
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发表时间：
2002-06-01
影响因子：
1.5
通讯作者：
Murakami-Murofushi, K
中科院分区：
文献类型：
--
作者：
Kunimoto, S;Murofushi, W;Murakami-Murofushi, K
我们以前报道过，暴露于环境压力下，steryl glucoside （SG）在霉菌细胞到人类细胞中被迅速诱导(murakami - murrofushi et al.（1997）。化学。Kunimoto et al. (2000) Cell Stress & Chaperones, 5,3 -7)，在霉菌细胞中，SG的产生伴随着某种蛋白激酶的激活和热休克蛋白（HSP）的诱导(Maruya et al. (1997) Cell Struct。功能。科学通报，21,533-538)。为了确定SG在应激反应信号转导中的生物学意义，我们将SG添加到人成纤维细胞中，并检测其对热休克蛋白诱导的影响。我们证明了热休克转录因子1 （HSF1）与热休克元件（HSE）结合的快速激活和热休克蛋白70 （HSP70）的诱导，通过暴露于外源性添加的人主要SG，胆固醇糖苷（CG）。此外，在成纤维细胞匀浆中检测了从胆固醇和udp -葡萄糖形成CG的酶活性。这些结果强烈表明，CG在应激反应信号转导的早期阶段起中介作用。
We previously reported that steryl glucoside (SG) is rapidly induced in cells from molds to humans by exposure to environmental stress (Murakami-Murofushi et al. (1997) J. Biol. Chem., 272, 486-489, Kunimoto et al. (2000) Cell Stress & Chaperones, 5, 3-7), and in mold cells SG production is followed by activation of a certain protein kinase and induction of heat shock proteins (HSP) (Maruya et al. (1997) Cell Struct. Funct., 21, 533-538). To determine the biological significance of SG in stress responsive signal transduction, we added SG to the culture of human fibroblasts and examined its effect on HSP induction. We demonstrated a rapid activation of heat shock transcription factor 1 (HSF1) to bind to heat shock element (HSE) and induction of heat shock protein 70 (HSP70) in fibroblast cells by exposure to exogenously added human major SG, cholesteryl glucoside (CG). In addition, enzyme activity to form CG from cholesterol and UDP-glucose was detected in the homogenate of fibroblast cells. These results strongly suggest that CG acts as a mediator in the early stage of stress responsive signal transduction.