Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons
Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons
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DOI：
10.1016/s0960-9822(02)01239-3
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发表时间：
2002-10-29
期刊：
影响因子：
9.2
通讯作者：
Galizia, CG
中科院分区：
文献类型：
--
作者：
Fiala, A;Spall, T;Galizia, CG
复杂的外部刺激，如气味，被认为在大脑中由广泛的神经元集合的时空活动模式在内部表示。这些活动模式可以通过光学成像技术记录下来。然而，使用常规荧光染料的光学成像通常不允许分辨生物定义的神经元组的活动。因此，将报告分子定位于具有共同遗传同一性的神经元群体是一个重要的目标。我们报告了遗传编码的钙敏感荧光蛋白Cameleon 2.1[1]在果蝇大脑中的应用。我们在选择性标记的嗅觉投射神经元中可视化了气味引起的细胞内钙浓度的变化，这些神经元在触角叶、初级嗅神经丛的突触后，以及在参与嗅觉学习和记忆的结构--蘑菇体花冠--的突触前。作为一项技术成就，我们证明了在活体大脑中使用基因编码的荧光探针进行钙成像是可行的。这将使人们能够将果蝇先进的遗传工具与大脑功能的生理分析结合起来。此外，我们还首次报道了果蝇蘑菇体花冠和触角叶突触区域的光学成像记录。这为利用果蝇作为嗅觉模型系统提供了重要的一步。
Complex external stimuli such as odorants are believed to be internally represented in the brain by spatiotemporal activity patterns of extensive neuronal ensembles. These activity patterns can be recorded by optical imaging techniques. However, optical imaging with conventional fluorescence dyes usually does not allow for resolving the activity of biologically defined groups of neurons. Therefore, specifically targeting reporter molecules to neuron populations of common genetic identity is an important goal. We report the use of the genetically encoded calcium-sensitive fluorescence protein cameleon 2.1 [1] in the Drosophila brain. We visualized odorant-evoked intracellular calcium concentration changes in selectively labeled olfactory projection neurons both postsynaptically in the antennal lobe, the primary olfactory neuropil, and presynaptically in the mushroom body calyx, a structure involved in olfactory learning and memory. As a technical achievement, we show that calcium imaging with a genetically encoded fluorescence probe is feasible in a brain in vivo. This will allow one to combine Drosophila's advanced genetic tools with the physiological analysis of brain function. Moreover, we report for the first time optical imaging recordings in synaptic regions of the Drosophila mushroom body calyx and antennal lobe. This provides an important step for the use of Drosophila as a model system in olfaction.